ArticleThe British journal of ophthalmology2008
Use of multiplex PCR and real-time PCR to detect human herpes virus genome in ocular fluids of patients with uveitis.
Article in The British journal of ophthalmology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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Who cites it
98 citing papers in PubMed, 231 citations in OpenAlex.
- Comprehensive polymerase chain reaction assay for detection of pathogenic DNA in lymphoproliferative disorders of the ocular adnexa.Scientific reports · 2016Trial
- Metagenomic next-generation sequencing for comprehensive pathogen detection in intraocular infection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Long-term structural impact and antiviral efficacy of cytomegalovirus anterior uveitis - results from a 10-year ocular inflammation registry.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2026Article
- Two Cases of Posner-Schlossman Syndrome With the Detection of Human Herpesvirus 6 DNA in the Aqueous Humor.Cureus · 2026Article
- Acute Retinal Necrosis in an Infant With Corneal and Macular Scarring.Journal of vitreoretinal diseases · 2026Article
- Quantitative Analysis of Background Intraocular Pressure Volatility and Retinal Nerve Fiber Layer Loss in Cytomegalovirus Anterior Uveitis.Translational vision science & technology · 2026Article
- Cytomegalovirus-Specific T Cell Immunity and Clinical Associations in Patients With CMV Anterior Uveitis.Investigative ophthalmology & visual science · 2026Article
- Clinical Assessment of a Highly Multiplexed Panel Assay for the Diagnosis of Infectious Uveitis.American journal of ophthalmology · 2026Article
- Low-volume multiplex solid-phase PCR without DNA extraction for etiological diagnosis of infectious uveitis.Indian journal of ophthalmology · 2026Article
- The Use of CSF Multiplex PCR Panel in Patients with Viral Uveitis.Diagnostics (Basel, Switzerland) · 2026Article
- Human herpesvirus 7 epithelial keratitis with geographic-dendritic ulceration.BMC infectious diseases · 2025Article
- PCR-based detection of viral and parasitic etiologies in anterior uveitis: a cross-sectional study from Iran.BMC ophthalmology · 2025Article
- An Unusual Manifestation of HSV-1 Uveitis Transforming into an Acute Iris Transillumination-like Syndrome with Pigmentary Glaucoma: A Reminder of Treatment Pitfalls in Herpetic Uveitis.Life (Basel, Switzerland) · 2025Article
- Comparative analysis of iridian anterior segment OCT and microbiological features in Fuchs Uveitis Syndrome and Posner-Schlossman Syndrome.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2025Article
- A Case of Branch Retinal Artery Occlusion With Concomitant Intraocular and Extraocular Toxoplasmosis Lesions in a Japanese Man.Case reports in ophthalmological medicine · 2025Article
- Next-generation sequencing and immuno-informatics for designing a multi-epitope vaccine against HSV-1-induced uveitis.Frontiers in immunology · 2025Article
- Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis.Journal of ophthalmic inflammation and infection · 2024Article
- Etiological analysis of ocular herpes virus infection.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2024Article
- Automated early detection of acute retinal necrosis from ultra-widefield color fundus photography using deep learning.Eye and vision (London, England) · 2024Article
- Article
38 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo measure the genomic DNA of human herpes viruses (HHV) in the ocular fluids and to analyse the clinical relevance of HHV in uveitis.
methodsAfter informed consent was obtained, a total of 111 ocular fluid samples (68 aqueous humour and 43 vitreous fluid samples) were collected from 100 patients with uveitis. The samples were assayed for HHV-DNA (HHV1-8) by using two different polymerase chain reaction (PCR) assays, qualitative PCR (multiplex PCR) and quantitative PCR (real-time PCR).
resultsIn all of the patients with acute retinal necrosis (n = 16) that were tested, either the HSV1 (n = 2), HSV2 (n = 3), or VZV (n = 11) genome was detected. In all patients, high copy numbers of the viral DNA were also noted, indicating the presence of viral replication. In another 10 patients with anterior uveitis with iris atrophy, the VZV genome was detected. When using multiplex PCR, EBV-DNA was detected in 19 of 111 samples (17%). However, real-time PCR analysis of EBV-DNA indicated that there were only six of the 19 samples that had significantly high copy numbers. The cytomegalovirus (CMV) genome was detected in three patients with anterior uveitis of immunocompetent patients and in one immunocompromised CMV retinitis patient. In addition, one patient with severe unilateral panuveitis had a high copy number of HHV6-DNA. There was no HHV7- or HHV8-DNA detected in any of the samples.
conclusionsA qualitative multiplex PCR is useful in the screening of viral infections. However, the clinical relevance of the virus infection needs to be evaluated by quantitative real-time PCR.
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