ArticleApplied and environmental microbiology2008
The probiotic Lactobacillus johnsonii NCC 533 produces high-molecular-mass inulin from sucrose by using an inulosucrase enzyme.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 102 citations in OpenAlex.
- Development and Characterization of Clindamycin-Loaded Dextran Hydrogel for Controlled Drug Release and Pathogen Inhibition.Gels (Basel, Switzerland) · 2026Article
- Improving the activity of an inulosucrase by rational engineering for the efficient biosynthesis of low-molecular-weight inulin.Archives of microbiology · 2024Article
- Improving the thermostability and modulating the inulin profile of inulosucrase through rational glycine-to-proline substitution.RSC advances · 2024Article
- Functional and genomic characterization of a novel probiotic Lactobacillus johnsonii KD1 against shrimp WSSV infection.Scientific reports · 2023Article
- New progress in the identifying regulatory factors of exopolysaccharide synthesis in lactic acid bacteria.World journal of microbiology & biotechnology · 2023Review
- Fructan Biosynthesis by Yeast Cell Factories.Journal of microbiology and biotechnology · 2022Review
- High-yield production and purification of prebiotic inulin-type fructooligosaccharides.AMB Express · 2022Article
- Production of fructan synthesis/hydrolysis of endophytic bacteria involved in inulin production in Jerusalem artichoke.3 Biotech · 2022Article
- Production of a high molecular weight levan by Bacillus paralicheniformis, an industrially and agriculturally important isolate from the buffalo grass rhizosphere.Antonie van Leeuwenhoek · 2022Article
- Structure-Function Relationship Studies of Multidomain Levansucrases from Leuconostocaceae Family.Microorganisms · 2022Article
- Characterization of a Novel Fructosyltransferase from Lactobacillus crispatus, InuCA, That Attaches to the Cell Surface by Electrostatic Interaction.Applied and environmental microbiology · 2022Article
- Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut.Folia microbiologica · 2022Article
- Cloning, Expression and Characterization of UDP-Glucose Dehydrogenases.Life (Basel, Switzerland) · 2021Article
- Spinal Cord Injury Changes the Structure and Functional Potential of Gut Bacterial and Viral Communities.mSystems · 2021Article
- Conserved Calcium-Binding Residues at the Ca-I Site Involved in Fructooligosaccharide Synthesis byACS omega · 2020Article
- Extracellular polysaccharides produced by bacteria of the Leuconostoc genus.World journal of microbiology & biotechnology · 2020Review
- Cross-Feeding among Probiotic Bacterial Strains on Prebiotic Inulin Involves the ExtracellularApplied and environmental microbiology · 2018Article
- Highly porous core-shell chitosan beads with superb immobilization efficiency forRSC advances · 2018Article
- Interactions of Surface Exopolysaccharides FromFrontiers in microbiology · 2018Review
- Synthesis of Fructooligosaccharides by IslA4, a truncated inulosucrase from Leuconostoc citreum.BMC biotechnology · 2015Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Fructansucrase enzymes polymerize the fructose moiety of sucrose into levan or inulin fructans, with beta(2-6) and beta(2-1) linkages, respectively. The probiotic bacterium Lactobacillus johnsonii strain NCC 533 possesses a single fructansucrase gene (open reading frame AAS08734) annotated as a putative levansucrase precursor. However, (13)C nuclear magnetic resonance (NMR) analysis of the fructan product synthesized in situ revealed that this is of the inulin type. The ftf gene of L. johnsonii was cloned and expressed to elucidate its exact identity. The purified L. johnsonii protein was characterized as an inulosucrase enzyme, producing inulin from sucrose, as identified by (13)C NMR analysis. Thin-layer chromatographic analysis of the reaction products showed that InuJ synthesized, besides the inulin polymer, a broad range of fructose oligosaccharides. Maximum InuJ enzyme activity was observed in a pH range of 4.5 to 7.0, decreasing sharply at pH 7.5. InuJ exhibited the highest enzyme activity at 55 degrees C, with a drastic decrease at 60 degrees C. Calcium ions were found to have an important effect on enzyme activity and stability. Kinetic analysis showed that the transfructosylation reaction of the InuJ enzyme does not obey Michaelis-Menten kinetics. The non-Michaelian behavior of InuJ may be attributed to the oligosaccharides that were initially formed in the reaction and which may act as better acceptors than the growing polymer chain. This is only the second example of the isolation and characterization of an inulosucrase enzyme and its inulin (oligosaccharide) product from a Lactobacillus strain. Furthermore, this is the first Lactobacillus strain shown to produce inulin polymer in situ.
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