ArticleJournal of bacteriology2008
Characterization of abn2 (yxiA), encoding a Bacillus subtilis GH43 arabinanase, Abn2, and its role in arabino-polysaccharide degradation.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Exo- and Endo-1,5-α-L-Arabinanases and Prebiotic Arabino-Oligosaccharides Production.Journal of microbiology and biotechnology · 2025Review
- Functional Characterization of Endo- and Exo-Hydrolase Genes in Arabinan Degradation Gene Cluster ofInternational journal of molecular sciences · 2024Article
- Arabinan hydrolysis by GH43 enzymes of Hungateiclostridium clariflavum and the potential synergistic mechanisms.Applied microbiology and biotechnology · 2022Article
- Engineering Caldicellulosiruptor bescii with Surface Layer Homology Domain-Linked Glycoside Hydrolases Improves Plant Biomass Solubilization.Applied and environmental microbiology · 2022Article
- Metabolic engineering enables Bacillus licheniformis to grow on the marine polysaccharide ulvan.Microbial cell factories · 2022Article
- Chemical and nutritional characteristics, and microbial degradation of rapeseed meal recalcitrant carbohydrates: A review.Frontiers in nutrition · 2022Review
- Characterization of two extracellular arabinanases in Lactobacillus crispatus.Applied microbiology and biotechnology · 2020Article
- Quantification and biodegradability assessment of meso-2,3-dimercaptosuccinic acid adsorbed on iron oxide nanoparticles.Nanoscale advances · 2019Article
- TheJournal of bacteriology · 2019Article
- Enzymatic Mechanism for Arabinan Degradation and Transport in the Thermophilic Bacterium Caldanaerobius polysaccharolyticus.Applied and environmental microbiology · 2017Article
- Article
- Role of the ganSPQAB Operon in Degradation of Galactan by Bacillus subtilis.Journal of bacteriology · 2016Article
- The LacI-Type transcriptional regulator AraR acts as an L-arabinose-responsive repressor of L-arabinose utilization genes in Corynebacterium glutamicum ATCC 31831.Journal of bacteriology · 2014Article
- The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2014Article
- Expression and characterization of a GH43 endo-arabinanase from Thermotoga thermarum.BMC biotechnology · 2014Article
- Mechanistic strategies for catalysis adopted by evolutionary distinct family 43 arabinanases.The Journal of biological chemistry · 2014Article
- Screening of thermotolerant and thermophilic fungi aiming β-xylosidase and arabinanase production.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Mining for hemicellulases in the fungus-growing termite Pseudacanthotermes militaris using functional metagenomics.Biotechnology for biofuels · 2013Article
- Detailed modes of action and biochemical characterization of endo-arabinanase from Bacillus licheniformis DSM13.Journal of microbiology (Seoul, Korea) · 2012Article
- The L-Arabinan utilization system of Geobacillus stearothermophilus.Journal of bacteriology · 2011Article
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2 authors at 1 institution in 1 country.
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Abstract
The extracellular depolymerization of arabinopolysaccharides by microorganisms is accomplished by arabinanases, xylanases, and galactanases. Here, we characterize a novel endo-alpha-1,5-l-arabinanase (EC 3.2.1.99) from Bacillus subtilis, encoded by the yxiA gene (herein renamed abn2) that contributes to arabinan degradation. Functional studies by mutational analysis showed that Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in B. subtilis. Abn2 was overproduced in Escherichia coli, purified from the periplasmic fraction, and characterized with respect to substrate specificity and biochemical and physical properties. With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50 degrees C. RNA studies revealed the monocistronic nature of abn2. Two potential transcriptional start sites were identified by primer extension analysis, and both a sigma(A)-dependent and a sigma(H)-dependent promoter were located. Transcriptional fusion studies revealed that the expression of abn2 is stimulated by arabinan and pectin and repressed by glucose; however, arabinose is not the natural inducer. Additionally, trans-acting factors and cis elements involved in transcription were investigated. Abn2 displayed a control mechanism at a level of gene expression different from that observed with AbnA. These distinct regulatory mechanisms exhibited by two members of extracellular glycoside hydrolase family 43 (GH43) suggest an adaptative strategy of B. subtilis for optimal degradation of arabinopolysaccharides.
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