ArticleExperimental eye research2008
Lengsin expression and function during zebrafish lens formation.
Article in Experimental eye research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Contributions of aquatic organisms to understanding the vertebrate ocular lens.Frontiers in ophthalmology · 2026Review
- Ten Candidate Genes Were Identified to Be Associated with the Great Growth Differentiation in the Three-Way Cross Hybrid Abalone.Animals : an open access journal from MDPI · 2025Article
- Transgenic Overexpression of Myocilin Leads to Variable Ocular Anterior Segment and Retinal Alterations Associated with Extracellular Matrix Abnormalities in Adult Zebrafish.International journal of molecular sciences · 2022Article
- Article
- Loss of αB-crystallin function in zebrafish reveals critical roles in the development of the lens and stress resistance of the heart.The Journal of biological chemistry · 2018Article
- Proteome Profiling of Developing Murine Lens Through Mass Spectrometry.Investigative ophthalmology & visual science · 2018Article
- Changes in zebrafish (Danio rerio) lens crystallin content during development.Molecular vision · 2013Article
- Integrin α5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish.Molecular biology of the cell · 2012Article
- The visual system of zebrafish and its use to model human ocular diseases.Developmental neurobiology · 2012Review
- Dynamic reorganization of metabolic enzymes into intracellular bodies.Annual review of cell and developmental biology · 2012Review
- Phosphatidylinositol synthase is required for lens structural integrity and photoreceptor cell survival in the zebrafish eye.Experimental eye research · 2011Article
- Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens.Developmental biology · 2011Article
- Toward a better understanding of human eye disease insights from the zebrafish, Danio rerio.Progress in molecular biology and translational science · 2011Review
- Two distinct aquaporin 0s required for development and transparency of the zebrafish lens.Investigative ophthalmology & visual science · 2010Article
- The zebrafish lens proteome during development and aging.Molecular vision · 2009Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
A zebrafish ortholog of human lengsin was identified by EST analysis of an adult lens cDNA library. During zebrafish development, lengsin transcription is first detected at 24 h post-fertilization (hpf). Immunolocalization, using polyclonal antiserum generated against a Lengsin bacterial fusion protein, detects lens-specific protein in whole-mount embryos at 30 hpf. Lengsin expression in zebrafish follows the temporal expression of the alphaA- alphaB1- and betaB1-crystallin proteins in the lens. At 72 hpf, Lengsin is localized to a subpopulation of differentiating secondary fiber cells, while no expression is detected in the lens epithelial cells or central lens fibers. In the adult lens, Lengsin is restricted to a narrow band of cortical fibers and co-localizes with actin at the lateral faces of these interdigitating cells. Stable transgenic lines, using a 3 kb lengsin genomic fragment to regulate EGFP expression, recapitulate the Lengsin temporal and spatial expression patterns. Lengsin function in zebrafish lens formation was examined by antisense morpholino-mediated translation and mRNA splice inhibition. At 72 hpf, the lengsin morphant lenses are reduced in size and exhibit separations within the cortex due to defects in secondary fiber morphogenesis. The location of the morphant lens defects correlates with the Lengsin protein localization at this age. These results demonstrate Lengsin is required for proper fiber cell differentiation by playing roles in either cell elongation or the establishment of cell interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.