ArticleInsect biochemistry and molecular biology2008
Cellular and molecular characterization of an embryonic cell line (BME26) from the tick Rhipicephalus (Boophilus) microplus.
Article in Insect biochemistry and molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 80 citations in OpenAlex.
- Unraveling the Roles of Epigenetic Regulators During the Embryonic Development ofInternational journal of molecular sciences · 2025Article
- Starvation Metabolism Adaptations in Tick Embryonic Cells BME26.International journal of molecular sciences · 2024Article
- Validation of heat-inducibleiScience · 2024Article
- Editorial: ticks & tick-borne parasites and diseases.Parasitology · 2024Article
- Aurora kinase as a putative target to tick control.Parasitology · 2024Article
- Phenotypic and Target-Directed Screening Yields New Acaricidal Alternatives for the Control of Ticks.Molecules (Basel, Switzerland) · 2022Article
- Pyrokinin receptor silencing in females of the southern cattle tick Rhipicephalus (Boophilus) microplus is associated with a reproductive fitness cost.Parasites & vectors · 2022Article
- Three-Dimensional Culture ofInsects · 2021Article
- Review
- The intracellular bacterium Rickettsia rickettsii exerts an inhibitory effect on the apoptosis of tick cells.Parasites & vectors · 2020Article
- The identification of tick autophagy-related genes in Ixodes scapularis responding to amino acid starvation.Ticks and tick-borne diseases · 2020Article
- Tick Cell Lines in Research on Tick Control.Frontiers in physiology · 2020Review
- Carbohydrate Metabolic Compensation Coupled to High Tolerance to Oxidative Stress in Ticks.Scientific reports · 2019Article
- TOR as a Regulatory Target inFrontiers in physiology · 2019Article
- Viral diversity of Rhipicephalus microplus parasitizing cattle in southern Brazil.Scientific reports · 2018Article
- Novel and SelectiveVeterinary sciences · 2018Article
- The Tick Cell Biobank: A global resource for in vitro research on ticks, other arthropods and the pathogens they transmit.Ticks and tick-borne diseases · 2018Review
- Immune-related redox metabolism of embryonic cells of the tick Rhipicephalus microplus (BME26) in response to infection with Anaplasma marginale.Parasites & vectors · 2017Article
- Relative transcription of autophagy-related genes in Amblyomma sculptum and Rhipicephalus microplus ticks.Experimental & applied acarology · 2017Article
- DecipheringFrontiers in cellular and infection microbiology · 2017Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
The cellular and molecular characteristics of a cell line (BME26) derived from embryos of the cattle tick Rhipicephalus (Boophilus) microplus were studied. The cells contained glycogen inclusions, numerous mitochondria, and vesicles with heterogeneous electron densities dispersed throughout the cytoplasm. Vesicles contained lipids and sequestered palladium meso-porphyrin (Pd-mP) and rhodamine-hemoglobin, suggesting their involvement in the autophagic and endocytic pathways. The cells phagocytosed yeast and expressed genes encoding the antimicrobial peptides (microplusin and defensin). A cDNA library was made and 898 unique mRNA sequences were obtained. Among them, 556 sequences were not significantly similar to any sequence found in public databases. Annotation using Gene Ontology revealed transcripts related to several different functional classes. We identified transcripts involved in immune response such as ferritin, serine proteases, protease inhibitors, antimicrobial peptides, heat shock protein, glutathione S-transferase, peroxidase, and NADPH oxidase. BME26 cells transfected with a plasmid carrying a red fluorescent protein reporter gene (DsRed2) transiently expressed DsRed2 for up to 5 weeks. We conclude that BME26 can be used to experimentally analyze diverse biological processes that occur in R. (B.) microplus such as the innate immune response to tick-borne pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.