ArticlePurinergic signalling2005
Release and extracellular metabolism of ATP by ecto-nucleotidase eNTPDase 1-3 in hypothalamic and pituitary cells.
Article in Purinergic signalling, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Cell Type-Specific Expression of Purinergic P2X Receptors in the Hypothalamus.International journal of molecular sciences · 2025Review
- Synthesis of new class of indole acetic acid sulfonate derivatives as ectonucleotidases inhibitors.RSC advances · 2023Article
- Neurotransmitter receptors as signaling platforms in anterior pituitary cells.Molecular and cellular endocrinology · 2018Review
- Interactions of Pannexin1 channels with purinergic and NMDA receptor channels.Biochimica et biophysica acta. Biomembranes · 2018Review
- Adenine Nucleotides Control Proliferation In Vivo of Rat Retinal Progenitors by P2YMolecular neurobiology · 2017Article
- Ion Channels of Pituitary Gonadotrophs and Their Roles in Signaling and Secretion.Frontiers in endocrinology · 2017Review
- The F0F1 ATP Synthase Complex Localizes to Membrane Rafts in Gonadotrope Cells.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Purinergic signaling pathways in endocrine system.Autonomic neuroscience : basic & clinical · 2015Review
- Purinergic signalling in endocrine organs.Purinergic signalling · 2014Review
- P2X receptor channels in endocrine glands.Wiley interdisciplinary reviews. Membrane transport and signaling · 2013Article
- P2X purinergic receptor knockout mice reveal endogenous ATP modulation of both vasopressin and oxytocin release from the intact neurohypophysis.Journal of neuroendocrinology · 2012Article
- Expression and roles of pannexins in ATP release in the pituitary gland.Endocrinology · 2011Article
- Expression of P2Y receptors in the rat anterior pituitary.Purinergic signalling · 2011Article
- Ion channels and signaling in the pituitary gland.Endocrine reviews · 2010Review
- Characterization of purinergic P2X4 receptor channels expressed in anterior pituitary cells.American journal of physiology. Endocrinology and metabolism · 2010Article
- Signaling by purinergic receptors and channels in the pituitary gland.Molecular and cellular endocrinology · 2010Review
- Purinergic regulation of hypothalamopituitary functions.Trends in endocrinology and metabolism: TEM · 2009Review
- Paracrinicity: the story of 30 years of cellular pituitary crosstalk.Journal of neuroendocrinology · 2008Review
- Specificity of the ecto-ATPase inhibitor ARL 67156 on human and mouse ectonucleotidases.British journal of pharmacology · 2007Article
- The E-NTPDase family of ectonucleotidases: Structure function relationships and pathophysiological significance.Purinergic signalling · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hypothalamic and pituitary cells express G protein-coupled adenosine and P2Y receptors and cation-conducting P2X receptor-channels, suggesting that extracellular ATP and other nucleotides may function as autocrine and/or paracrine signaling factors in these cells. Consistent with this hypothesis, we show that cultured normal and immortalized pituitary and hypothalamic cells release ATP under resting conditions. RT-PCR analysis also revealed the presence of transcripts for ecto-nucleotidase eNTPDase 1-3 in these cells. These enzymes were functional as documented by degradation of endogenously released and exogenously added ATP. Blocking the activity of eNTPDases by ARL67156 led to an increase in ATP release in perifused pituitary cells and inhibition of degradation of extracellularly added ATP. Furthermore, the addition of apyrase, a soluble ecto-nucleotidase, and the expression of recombinant mouse eNTPDase-2, enhanced degradation of both endogenously released and exogenously added ATP. The released ATP by resting hypothalamic cells was sufficient to activate and desensitize high-affinity recombinant P2X receptors, whereas facilitation of ATP metabolism by the addition of apyrase protected their desensitization. These results indicate that colocalization of ATP release sites and ecto-nucleotidase activity at the plasma membrane of hypothalamic and pituitary cells provides an effective mechanism for the operation of nucleotides as extracellular signaling molecules.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.