ArticlePurinergic signalling2006
Increased 5-HT(3)-mediated signalling in pelvic afferent neurons from mice deficient in P2X(2) and/or P2X (3) receptor subunits.
Article in Purinergic signalling, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 7 citations in OpenAlex.
- Molecular and functional properties of P2X receptors--recent progress and persisting challenges.Purinergic signalling · 2012Review
- Interaction of P2 purinergic receptors with cellular macromolecules.Naunyn-Schmiedeberg's archives of pharmacology · 2008Review
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular ATP and 5-hydroxytryptamine (5-HT) are both involved in visceral sensory pathways by interacting with P2X and 5-HT(3) receptors, respectively. We have investigated the changes in P2X and 5-HT(3)-mediated signalling in pelvic afferent neurons in mice deficient in P2X(2) and/or P2X(3) subunits by whole-cell recording of L(6)-S(2) dorsal root ganglion (DRG) neurons and by multi-unit recording of pelvic afferents of the colorectum. In wildtype DRG neurons, ATP evoked transient, sustained or mixed (biphasic) inward currents. Transient currents were absent in P2X(3) (-/-) neurons, whereas sustained currents were absent in P2X(2) (-/-) DRG neurons. Neither transient nor sustained currents were observed following application of ATP or alpha,beta-methylene ATP (alpha,beta-meATP) in P2X(2)/P2X(3) (Dbl-/-) DRG neurons. 5-HT was found to induce a fast inward current in 63% of DRG neurons from wildtype mice, which was blocked by tropisetron, a 5-HT(3) receptor antagonist. The percentage of DRG neurons responding to 5-HT was significantly increased in P2X (2) (-/-), P2X(3) (-/-) and P2X(2)/P2X(3) (Dbl-/-) mice, and the amplitude of 5-HT response was significantly increased in P2X(2)/P2X(3) (Dbl-/-) mice. The pelvic afferent response to colorectal distension was attenuated in P2X(2)/P2X(3) (Dbl-/-) mice, but the response to serosal application of 5-HT was enhanced. Furthermore, tropisetron resulted in a greater reduction in pelvic afferent responses to colorectal distension in the P2X(2)/P2X(3) (Dbl-/-) preparations. These data suggest that P2X receptors containing the P2X(2) and/or P2X(3) subunits mediate purinergic activation of colorectal afferents and that 5-HT signalling in pelvic afferent neurons is up-regulated in mice lacking P2X(2) or P2X(3) receptor genes. This effect is more pronounced when both subunits are absent.
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Registered trials
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