ArticleNucleic acids research2008
TFIIB aptamers inhibit transcription by perturbing PIC formation at distinct stages.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane.Nanomaterials (Basel, Switzerland) · 2020Article
- An RNA aptamer to HP1/Swi6 facilitates heterochromatin formation at an ectopic locus in S.pombe.RNA biology · 2019Article
- RNA aptamer inhibitors of a restriction endonuclease.Nucleic acids research · 2015Article
- New Technologies Provide Quantum Changes in the Scale, Speed, and Success of SELEX Methods and Aptamer Characterization.Molecular therapy. Nucleic acids · 2014Article
- General transcription factor IIb overexpression and a potential link to proliferation in human hepatocellular carcinoma.Pathology oncology research : POR · 2013Article
- RNA aptamers that functionally interact with green fluorescent protein and its derivatives.Nucleic acids research · 2012Article
- An RNA aptamer perturbs heat shock transcription factor activity in Drosophila melanogaster.Nucleic acids research · 2011Article
- An RNA-based transcription activator derived from an inhibitory aptamer.Nucleic acids research · 2010Article
- Riboactivators: transcription activation by noncoding RNA.Critical reviews in biochemistry and molecular biologyReview
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transcription in eukaryotes is a multistep process involving the assembly and disassembly of numerous inter- and intramolecular interactions between transcription factors and nucleic acids. The roles of each of these interactions and the regions responsible for them have been identified and studied primarily by the use of mutants, which destroy the inherent properties of the interacting surface. A less intrusive but potentially effective way to study the interactions as well as the surfaces responsible for them is the use of RNA aptamers that bind to the interacting factors. Here, we report the isolation and characterization of high-affinity RNA aptamers that bind to the yeast general transcription factor TFIIB. These aptamers fall into two classes that interfere with TFIIB's interactions with either TBP or RNA polymerase II, both of which are crucial for transcription in yeast. We demonstrate the high affinity and specificity of these reagents, their effect on transcription and preinitiation complex formation and discuss their potential use to address mechanistic questions in vitro as well as in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.