ReviewCurrent opinion in structural biology2008
Towards atomic resolution structural determination by single-particle cryo-electron microscopy.
Review in Current opinion in structural biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
69 citing papers in PubMed, 173 citations in OpenAlex.
- Isolation and Visualization Fusobacterium nucleatum Microcompartments.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Non-uniform Fourier transform based image classification in single-particle Cryo-EM.Journal of structural biology: X · 2025Article
- Three-dimensional electron ptychography of organic-inorganic hybrid nanostructures.Nature communications · 2022Article
- Gum-Net: Unsupervised Geometric Matching for Fast and Accurate 3D Subtomogram Image Alignment and Averaging.Proceedings. IEEE Computer Society Conference on Computer Vision and Pattern Recognition · 2020Article
- A comparative study of single-particle cryo-EM with liquid-nitrogen and liquid-helium cooling.IUCrJ · 2019Article
- Electrophoretic exclusion microscale sample preparation for cryo-EM structural determination of proteins.Biomicrofluidics · 2019Article
- Deep Learning for Validating and Estimating Resolution of Cryo-Electron Microscopy Density MapsMolecules (Basel, Switzerland) · 2019Article
- Recent advances in retroviruses via cryo-electron microscopy.Retrovirology · 2018Review
- Estimating the effect of finite depth of field in single-particle cryo-EM.Ultramicroscopy · 2018Article
- Modeling Beta-Traces for Beta-Barrels from Cryo-EM Density Maps.BioMed research international · 2017Article
- Nanostructured glycan architecture is important in the inhibition of influenza A virus infection.Nature nanotechnology · 2017Article
- An Iterative Bézier Method for Fitting Beta-sheet Component of a Cryo-EM Density Map.Molecular based mathematical biology · 2017Article
- 3D reconstruction of biological structures: automated procedures for alignment and reconstruction of multiple tilt series in electron tomography.Advanced structural and chemical imaging · 2017Article
- Long shelf-life streptavidin support-films suitable for electron microscopy of biological macromolecules.Journal of structural biology · 2016Article
- Diffraction Techniques in Structural Biology.Current protocols in nucleic acid chemistry · 2016Review
- Structural Insights into Reelin Function: Present and Future.Frontiers in cellular neuroscience · 2016Review
- Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells.Viruses · 2015Review
- Nature's favorite building block: Deciphering folding and capsid assembly of proteins with the HK97-fold.Virology · 2015Review
- De novo protein structure determination from near-atomic-resolution cryo-EM maps.Nature methods · 2015Article
- Visualizing transient dark states by NMR spectroscopy.Quarterly reviews of biophysics · 2015Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Recent advances in cryo-electron microscopy and single-particle reconstruction (collectively referred to as 'cryoEM') have made it possible to determine the three-dimensional (3D) structures of several macromolecular complexes at near-atomic resolution ( approximately 3.8-4.5A). These achievements were accomplished by overcoming the challenges in sample handling, instrumentation, image processing, and model building. At near-atomic resolution, many detailed structural features can be resolved, such as the turns and deep grooves of helices, strand separation in beta sheets, and densities for loops and bulky amino acid side chains. Such structural data of the cytoplasmic polyhedrosis virus (CPV), the Epsilon 15 bacteriophage and the GroEL complex have provided valuable constraints for atomic model building using integrative tools, thus significantly enhancing the value of the cryoEM structures. The CPV structure revealed a drastic conformational change from a helix to a beta hairpin associated with RNA packaging and replication, coupling of RNA processing and release, and the long sought-after polyhedrin-binding domain. These latest advances in single-particle cryoEM provide exciting opportunities for the 3D structural determination of viruses and macromolecular complexes that are either too large or too heterogeneous to be investigated by conventional X-ray crystallography or nuclear magnetic resonance (NMR) methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.