ArticleNeurochemical research2008
Enhanced trafficking of tetrameric Kv4.3 channels by KChIP1 clamping.
Article in Neurochemical research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- KChIP1 splice variants modulate Kv4 channels by promoting P/C-type inactivation features.Scientific reports · 2026Article
- Induction of antiviral interferon-stimulated genes by neuronal STING promotes the resolution of pain in mice.The Journal of clinical investigation · 2024Article
- Cellular and Subcellular Localisation of Kv4-Associated KChIP Proteins in the Rat Cerebellum.International journal of molecular sciences · 2020Article
- Somatodendritic surface expression of epitope-tagged and KChIP binding-deficient Kv4.2 channels in hippocampal neurons.PloS one · 2018Article
- Different KChIPs compete for heteromultimeric assembly with pore-forming Kv4 subunits.Biophysical journal · 2015Article
- Modulation of the voltage-gated potassium channel (Kv4.3) and the auxiliary protein (KChIP3) interactions by the current activator NS5806.The Journal of biological chemistry · 2014Article
- Article
- CaMKII regulation of cardiac K channels.Frontiers in pharmacology · 2014Review
- Kv4.2 potassium channels segregate to extrasynaptic domains and influence intrasynaptic NMDA receptor NR2B subunit expression.Brain structure & function · 2013Article
- Functional stoichiometry underlying KChIP regulation of Kv4.2 functional expression.Journal of neurochemistry · 2013Article
- Auxiliary KChIP4a suppresses A-type K+ current through endoplasmic reticulum (ER) retention and promoting closed-state inactivation of Kv4 channels.The Journal of biological chemistry · 2013Article
- Negative modulation of NMDA receptor channel function by DREAM/calsenilin/KChIP3 provides neuroprotection?Frontiers in molecular neuroscience · 2012Article
- Expression and distribution of Kv4 potassium channel subunits and potassium channel interacting proteins in subpopulations of interneurons in the basolateral amygdala.Neuroscience · 2010Article
- Functional rescue of Kv4.3 channel tetramerization mutants by KChIP4a.Biophysical journal · 2010Article
- Convergent modulation of Kv4.2 channel alpha subunits by structurally distinct DPPX and KChIP auxiliary subunits.Biochemistry · 2009Article
- Weighing the evidence for a ternary protein complex mediating A-type K+ currents in neurons.The Journal of physiology · 2008Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytoplamsic auxiliary KChIPs modulate surface expression and gating properties of Kv4 channels. Recent co-crystal structure of Kv4.3 N-terminus and KChIP1 reveals a clamping action of the complex in which a single KChIP1 molecule laterally binds two neighboring Kv4.3 N-termini at different locations, thus forming two contact interfaces involved in the protein-protein interaction. In the second interface, it functions to stabilize the tetrameric assembly, but the role it plays in channel trafficking remains elusive. In this study, we examined the effects of KChIP1 on Kv4 protein trafficking in COS-7 cells expressing EGFP-tagged Kv4.3 channels using confocal microscopy. Mutations either in KChIP1 (KChIP1 L39E-Y57A-K61A) or Kv4.3 (Kv4.3 E70A-F73E) that disrupt the protein-protein interaction within the second interface can reduce surface expression of Kv4 channel proteins. Kv4.3 C110A, the Zn2+ binding site mutation in T1 domain, that disrupts the tetrameric assembly of the channels can be rescued by WT KChIP1, but not the KChIP1 triple mutant. These results were further confirmed by whole cell current recordings in oocytes. Our findings show that key residues of second interface involved in stabilizing tetrameric assembly can regulate the channel trafficking, indicating an intrinsic link between tetrameric assembly and channel trafficking. The results also suggest that formation of octameric Kv4 and KChIP complex by KChIPs clamping takes place before their trafficking to final destination on the cell surface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.