ArticleIn vitro cellular & developmental biology. Animal
Cytological properties of an Aedes albopictus mosquito cell line infected with Wolbachia strain wAlbB.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Comparative tissue-specific microbiome analyses identify keystone endosymbionts shaping pathogen interactions in dromedary camel ticks.BMC microbiology · 2026Article
- Virus-like Particles fromInsects · 2023Article
- Mitotically inactivated mosquito cells support robust Wolbachia infection and replication.In vitro cellular & developmental biology. Animal · 2022Article
- From Mosquito Ovaries to Ecdysone; from Ecdysone toInsects · 2022Review
- Two Newly IntroducedApplied and environmental microbiology · 2021Article
- Growth and Maintenance ofInsects · 2021Review
- Conditions facilitating infection of mosquito cell lines with Wolbachia, an obligate intracellular bacterium.In vitro cellular & developmental biology. Animal · 2019Article
- Strain-specific response to ampicillin in Wolbachia-infected mosquito cell lines.In vitro cellular & developmental biology. Animal · 2018Article
- Proteomic analysis of a mosquito host cell response to persistent Wolbachia infection.Research in microbiology · 2017Article
- Mosaic composition of ribA and wspB genes flanking the virB8-D4 operon in the Wolbachia supergroup B-strain, wStr.Archives of microbiology · 2016Article
- Effects of mimosine on Wolbachia in mosquito cells: cell cycle suppression reduces bacterial abundance.In vitro cellular & developmental biology. Animal · 2015Article
- Flow cytometric evaluation of the intracellular bacterium, Wolbachia pipientis, in mosquito cells.Journal of microbiological methods · 2014Article
- Proteomic profiling of a robust Wolbachia infection in an Aedes albopictus mosquito cell line.Molecular microbiology · 2014Article
- Depletion of host cell riboflavin reduces Wolbachia levels in cultured mosquito cells.In vitro cellular & developmental biology. Animal · 2014Article
- The oxidizing agent, paraquat, is more toxic to Wolbachia than to mosquito host cells.In vitro cellular & developmental biology. Animal · 2013Article
- Wolbachia from the planthopper Laodelphax striatellus establishes a robust, persistent, streptomycin-resistant infection in clonal mosquito cells.In vitro cellular & developmental biology. Animal · 2013Article
- Decapitation improves detection of Wolbachia pipientis (Rickettsiales: Anaplasmataceae) in Culex pipiens (Diptera: Culicidae) mosquitoes by the polymerase chain reaction.Journal of medical entomology · 2012Article
- Proteasome activity in a naïve mosquito cell line infected with Wolbachia pipientis wAlbB.In vitro cellular & developmental biology. Animal · 2009Article
- Standardization of a colorimetric method to quantify growth and metabolic activity of Wolbachia-infected mosquito cells.In vitro cellular & developmental biology. AnimalArticle
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
In vitro production of the obligate intracellular bacterium, Wolbachia pipientis, is essential to its manipulation as a genetic tool to spread transgenes within vector populations. We have adapted the Wolbachia-infected Aa23 Aedes albopictus mosquito cell line to Eagle's minimal medium, supplemented with nonessential amino acids, glutamine, and 20% fetal bovine serum. When plated at low densities, Aa23E cells grew as patchy monolayers, comprised of non-contiguous clusters of cells that gave rise to solid clumps of tightly adherent cells. Multicellular clumps eventually detached from the substrate and floated freely in the medium. Removal of Wolbachia by treatment with tetracycline did not alter the cytological properties of the host cells, which had a population doubling time of 4-5 d. The presence of Wolbachia was monitored by Giemsa staining of cytological preparations, polymerase chain reaction (PCR) amplification of Wolbachia 16S ribosomal DNA, and by simultaneous PCR amplification of ribosomal protein genes from Wolbachia and mosquito host cell genomes. Wolbachia morphology was pleomorphic, and Wolbachia DNA persisted in the culture medium for several weeks after degradation of PCR-amplifiable host cell DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.