ArticleJournal of biomedicine & biotechnology2008
Competition of Lactobacillus paracasei with Salmonella enterica for adhesion to Caco-2 cells.
Article in Journal of biomedicine & biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 99 citations in OpenAlex.
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- Lacticaseibacillus paracasei subsp. paracasei Q- 1 Exhibits Good Safety and Effectively Prevents Escherichia coli K99-Induced Diarrhea in Mice.Probiotics and antimicrobial proteins · 2026Article
- Anti-invasion activities of heat-killed lactic acid bacteria isolates againstIranian journal of microbiology · 2024Article
- Evaluation of the Probiotic Properties ofFood technology and biotechnology · 2023Article
- FeedingAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Biochemical, functional and genomic characterization of a new probiotic Ligilactobacillus salivarius F14 from the gut of tribes of Odisha.World journal of microbiology & biotechnology · 2023Article
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- Lactobacilli, a Weapon to Counteract Pathogens through the Inhibition of Their Virulence Factors.Journal of bacteriology · 2022Review
- The Detection of Potential Native ProbioticsAntibiotics (Basel, Switzerland) · 2022Article
- Probiotic Properties and Immunomodulatory Activity ofMicroorganisms · 2021Article
- In Vitro Screening of Chicken-DerivedFoods (Basel, Switzerland) · 2021Article
- Cell-Free Lactobacillus sp Supernatants Modulate Staphylococcus aureus Growth, Adhesion and Invasion to Human Osteoblast (HOB) Cells.Current microbiology · 2021Article
- Anti-Salmonella Potential of NewPolish journal of microbiology · 2020Article
- Probiotic and Safety Properties Screening of Enterococcus faecalis from Healthy Chinese Infants.Probiotics and antimicrobial proteins · 2020Article
- Probiotic Characterization of Lactobacillus paracasei subsp. paracasei KNI9 Inhibiting Adherence of Yersinia enterocolitica on Caco-2 Cells In Vitro.Probiotics and antimicrobial proteins · 2020Article
- Evaluation of lactic acid bacteria isolated from poultry feces as potential probiotic and itsVeterinary research forum : an international quarterly journal · 2020Article
- Comparative genomics and functional analysis of a highly adhesive dairy Lactobacillus paracasei subsp. paracasei IBB3423 strain.Applied microbiology and biotechnology · 2019Article
- Lactobacillus casei expressing Internalins A and B reduces Listeria monocytogenes interaction with Caco-2 cells in vitro.Microbial biotechnology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Competition of commensal and probiotic bacteria with pathogens for adhesion and colonization is one of the important protective mechanisms of gastrointestinal tract. In this study, we examined the ability of Lactobacillus paracasei to inhibit the adhesion of pathogenic Salmonella enterica to human colon adenocarcinoma Caco-2 cells. Caco-2 cells were grown for 6 or 21 days to obtain nondifferentiated or well-differentiated cells, respectively. In adhesion experiments, bacteria were added to the cells for 2 or 4 hours. The number of attached bacteria was expressed as colony-forming units (CFUs), Caco-2 cells were counted in hematocytometer. Both bacterial strains used adhered better to well-differentiated than to nondifferentiated Caco-2 cells, however, the amount of Salmonella adhered to Caco-2 after 2 hours of contact was 12-fold higher in comparison to L. paracasei and almost 27-fold higher after 4 hours of contact. Two types of experiments were done: coincubation (both bacteria were added to Caco-2 cells simultaneously), and preincubation (L. paracasei was incubated with Caco-2 cells first, and then S. enterica was added). In coincubation experiment, the presence of L. paracasei decreased S. enterica adhesion by 4-fold and in preincubation experiment even 7-fold. Generally, Lactobacillus spent culture supernatants (SCSs) acted weaker as inhibitors of Salmonella adhesion in comparison to the whole L. paracasei culture in coincubation experiment. In conclusion, the displacement of pathogens by lactic acid bacteria and its secretions showed here depends on the time of bacteria-epithelial cell contact, and also on the stage of Caco-2 differentiation.
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