ArticleThe EMBO journal2008
A molecular switch required for retrovirus assembly participates in the hexagonal immature lattice.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 29 citations in OpenAlex.
- Effect of Small Polyanions on In Vitro Assembly of Selected Members of Alpha-, Beta- and Gammaretroviruses.Viruses · 2021Article
- Structures of immature EIAV Gag lattices reveal a conserved role for IP6 in lentivirus assembly.PLoS pathogens · 2020Article
- Recent advances in retroviruses via cryo-electron microscopy.Retrovirology · 2018Review
- Orchestrating the Selection and Packaging of Genomic RNA by Retroviruses: An Ensemble of Viral and Host Factors.Viruses · 2016Review
- Computational Methodologies for Real-Space Structural Refinement of Large Macromolecular Complexes.Annual review of biophysics · 2016Review
- Membrane Binding of the Rous Sarcoma Virus Gag Protein Is Cooperative and Dependent on the Spacer Peptide Assembly Domain.Journal of virology · 2015Article
- Hydrodynamic and Membrane Binding Properties of Purified Rous Sarcoma Virus Gag Protein.Journal of virology · 2015Article
- The Structure of Immature Virus-Like Rous Sarcoma Virus Gag Particles Reveals a Structural Role for the p10 Domain in Assembly.Journal of virology · 2015Article
- Atomic Modeling of an Immature Retroviral Lattice Using Molecular Dynamics and Mutagenesis.Structure (London, England : 1993) · 2015Article
- Stabilization of the β-hairpin in Mason-Pfizer monkey virus capsid protein- a critical step for infectivity.Retrovirology · 2014Article
- Higher-order structure of the Rous sarcoma virus SP assembly domain.Journal of virology · 2014Article
- Avian oncogenesis induced by lymphoproliferative disease virus: a neglected or emerging retroviral pathogen?Virology · 2014Article
- Effect of multimerization on membrane association of Rous sarcoma virus and HIV-1 matrix domain proteins.Journal of virology · 2013Article
- CRM1-dependent trafficking of retroviral Gag proteins revisited.Journal of virology · 2012Article
- In vitro assembly of virus-like particles of a gammaretrovirus, the murine leukemia virus XMRV.Journal of virology · 2012Article
- Assembly and architecture of HIV.Advances in experimental medicine and biology · 2012Review
- Design of in vitro symmetric complexes and analysis by hybrid methods reveal mechanisms of HIV capsid assembly.Journal of molecular biology · 2011Article
- Rhesus TRIM5α disrupts the HIV-1 capsid at the inter-hexamer interfaces.PLoS pathogens · 2011Article
- Structure of the HIV-1 full-length capsid protein in a conformationally trapped unassembled state induced by small-molecule binding.Journal of molecular biology · 2011Article
- The noncanonical Gag domains p8 and n are critical for assembly and release of mouse mammary tumor virus.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the Rous sarcoma virus (RSV) Gag protein, the 25 amino-acid residues of the p10 domain immediately upstream of the CA domain are essential for immature particle formation. We performed systematic mutagenesis on this region and found excellent correlation between the amino-acid side chains required for in vitro assembly and those that participate in the p10-CA dimer interface in a previously described crystal structure. We introduced exogenous cysteine residues that were predicted to form disulphide bonds across the dimer interface. Upon oxidation of immature particles, a disulphide-linked Gag hexamer was formed, implying that p10 participates in and stabilizes the immature Gag hexamer. This is the first example of a critical interaction between two different Gag domains. Molecular modeling of the RSV immature hexamer indicates that the N-terminal domains of CA must expand relative to the murine leukaemia virus mature hexamer to accommodate the p10 contact; this expansion is strikingly similar to recent cryotomography results for immature human immunodeficiency virus particles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.