ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2008
Connexin 40 and ATP-dependent intercellular calcium wave in renal glomerular endothelial cells.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 68 citations in OpenAlex.
- Skin capillary endothelial cells form a network of spatiotemporally conserved CaProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Skin capillary endothelial cells form a network of spatiotemporally conserved CabioRxiv : the preprint server for biology · 2026Article
- Macula densa cell angiogenic mechanisms and their therapeutic potential in kidney disease.Frontiers in bioengineering and biotechnology · 2025Article
- Calcium Regulation of Connexin Hemichannels.International journal of molecular sciences · 2024Review
- Angiotensin II Directly Increases Endothelial Calcium and Nitric Oxide in Kidney and Brain Microvessels In Vivo With Reduced Efficacy in Hypertension.Journal of the American Heart Association · 2024Article
- Calcium Signaling in the Photodamaged Skin: In Vivo Experiments and Mathematical Modeling.Function (Oxford, England) · 2022Article
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- Ablation of both Cx40 and Panx1 results in similar cardiovascular phenotypes exhibited in Cx40 knockout mice.Bioscience reports · 2019Article
- A Novel Role of Connexin 40-Formed Channels in the Enhanced Efficacy of Photodynamic Therapy.Frontiers in oncology · 2019Article
- The Potential for Connexin Hemichannels to Drive Breast Cancer Progression through Regulation of the Inflammatory Response.International journal of molecular sciences · 2018Review
- Pannexin channel and connexin hemichannel expression in vascular function and inflammation.BMC cell biology · 2017Review
- Critical role of ATP-induced ATP release for Ca2+ signaling in nonsensory cell networks of the developing cochlea.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Extracellular Cysteine in Connexins: Role as Redox Sensors.Frontiers in physiology · 2016Article
- Renal autoregulation in health and disease.Physiological reviews · 2015Review
- ATP acting through P2Y receptors causes activation of podocyte TRPC6 channels: role of podocin and reactive oxygen species.American journal of physiology. Renal physiology · 2014Article
- Intravital imaging of podocyte calcium in glomerular injury and disease.The Journal of clinical investigation · 2014Article
- Purinergic signalling in the kidney in health and disease.Purinergic signalling · 2014Review
- P2 receptors in renal autoregulation.Current vascular pharmacology · 2014Review
- Distribution and functional relevance of connexins in renin-producing cells.Pflugers Archiv : European journal of physiology · 2013Review
- The influence of extracellular and intracellular calcium on the secretion of renin.Pflugers Archiv : European journal of physiology · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Endothelial intracellular calcium ([Ca(2+)](i)) plays an important role in the function of the juxtaglomerular vasculature. The present studies aimed to identify the existence and molecular elements of an endothelial calcium wave in cultured glomerular endothelial cells (GENC). GENCs on glass coverslips were loaded with Fluo-4/Fura red, and ratiometric [Ca(2+)](i) imaging was performed using fluorescence confocal microscopy. Mechanical stimulation of a single GENC caused a nine-fold increase in [Ca(2+)](i), which propagated from cell to cell throughout the monolayer (7.9 +/- 0.3 microm/s) in a regenerative manner (without decrement of amplitude, kinetics, and speed) over distances >400 microm. Inhibition of voltage-dependent calcium channels with nifedipine had no effect on the above parameters, but the removal of extracellular calcium reduced Delta[Ca(2+)](i) by 50%. Importantly, the gap junction uncoupler alpha-glycyrrhetinic acid or knockdown of connexin 40 (Cx40) by transfecting GENCs with Cx40 short interfering RNA (siRNA) almost completely eliminated Delta[Ca(2+)](i) and the calcium wave. Breakdown of extracellular ATP using a scavenger cocktail (apyrase and hexokinase) or nonselective inhibition of purinergic P2 receptors with suramin, had similar blocking effects. Scraping cells off along a line eliminated physical contact between cells but did not effect calcium wave propagation. Using an ATP biosensor technique, we detected a significant elevation in extracellular ATP (Delta = 76 +/- 2 microM) during calcium wave propagation, which was abolished by Cx40 siRNA treatment (Delta = 6 +/- 1 microM). These studies suggest that connexin 40 hemichannels and extracellular ATP are key molecular elements of the glomerular endothelial calcium wave, which may serve important juxtaglomerular functions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.