Evidence map›Paper›PMID 18400916›Full record

ArticleJournal of clinical microbiology2008

Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens.

Robert Benson, Maria L Tondella, Julu Bhatnagar, Maria da Glória S Carvalho, Jacquelyn S Sampson, Deborah F Talkington, Anne M Whitney, Elizabeth Mothershed, Lesley McGee, George Carlone and 7 more

Abstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2015
    Article
  7. Article
  8. What is the role of newer molecular tests in the management of CAP?Infectious disease clinics of North America · 2013
    Review
  9. Article
  10. Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study).European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012
    Article
  11. Review
  12. Use and evaluation of molecular diagnostics for pneumonia etiology studies.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012
    Review
  13. Article
  14. Are we ready for novel detection methods to treat respiratory pathogens in hospital-acquired pneumonia?Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2011
    Review
  15. Multiplex PCR and emerging technologies for the detection of respiratory pathogens.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2011
    Review
  16. Article
  17. Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010
    Review
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Robert BensonCDC, Atlanta, GA 30333, USA.
Maria L Tondella
Julu Bhatnagar
Maria da Glória S Carvalho
Jacquelyn S Sampson
Deborah F Talkington
Anne M Whitney
Elizabeth Mothershed
Lesley McGee
George Carlone
Vondguraus McClee
Jeannette Guarner
Sherif Zaki
Surang Dejsiri
K Cronin
Jian Han
Barry S Fields

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.

Indexed as

Reagent Kits, DiagnosticBacteriaBacterial InfectionsDNA, BacterialDNA PrimersHumansPolymerase Chain ReactionRespiratory SystemRespiratory Tract InfectionsSensitivity and SpecificityDNA, BacterialDNA PrimersReagent Kits, Diagnostic

Identifiers

PMID18400916
PMCPMC2446872

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.