ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2008
Perturbation of syndapin/PACSIN impairs synaptic vesicle recycling evoked by intense stimulation.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 65 citations in OpenAlex.
- Compartmentalization of soluble endocytic proteins in synaptic vesicle clusters by phase separation.iScience · 2023Article
- Genetically encoded intrabodies as high-precision tools to visualize and manipulate neuronal function.Seminars in cell & developmental biology · 2022Article
- PACSIN proteins in vivo: Roles in development and physiology.Acta physiologica (Oxford, England) · 2022Review
- α-Synuclein in the Synaptic Vesicle Liquid Phase: Active Player or Passive Bystander?Frontiers in molecular biosciences · 2022Review
- Synaptic vesicle generation from activity-dependent bulk endosomes requires a dephosphorylation-dependent dynamin-syndapin interaction.Journal of neurochemistry · 2019Article
- Loss of myosin Vb promotes apical bulk endocytosis in neonatal enterocytes.The Journal of cell biology · 2019Article
- BAR domain proteins-a linkage between cellular membranes, signaling pathways, and the actin cytoskeleton.Biophysical reviews · 2018Review
- Identification of methylation sites and signature genes with prognostic value for luminal breast cancer.BMC cancer · 2018Article
- Synaptic Vesicle Endocytosis in Different Model Systems.Frontiers in cellular neuroscience · 2018Review
- Syndapin/SDPN-1 is required for endocytic recycling and endosomal actin association in the C. elegans intestine.Molecular biology of the cell · 2016Article
- PACSIN1 regulates the dynamics of AMPA receptor trafficking.Scientific reports · 2016Article
- Acute increase of α-synuclein inhibits synaptic vesicle recycling evoked during intense stimulation.Molecular biology of the cell · 2014Article
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- ProSAP1 and membrane nanodomain-associated syndapin I promote postsynapse formation and function.The Journal of cell biology · 2014Article
- Proteomic analysis of glycine receptor β subunit (GlyRβ)-interacting proteins: evidence for syndapin I regulating synaptic glycine receptors.The Journal of biological chemistry · 2014Article
- PICK1 interacts with PACSIN to regulate AMPA receptor internalization and cerebellar long-term depression.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Roles of GSK3β in odor habituation and spontaneous neural activity of the mouse olfactory bulb.PloS one · 2013Article
- Synaptic vesicle endocytosis.Cold Spring Harbor perspectives in biology · 2012Review
- Ultrastructural freeze-fracture immunolabeling identifies plasma membrane-localized syndapin II as a crucial factor in shaping caveolae.Histochemistry and cell biology · 2012Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptic vesicle recycling has been proposed to depend on proteins which coordinate membrane and cytoskeletal dynamics. Here, we examine the role of the dynamin- and N-WASP (neural Wiskott-Aldrich syndrome protein)-binding protein syndapin/PACSIN at the lamprey reticulospinal synapse. We find that presynaptic microinjection of syndapin antibodies inhibits vesicle recycling evoked by intense (5 Hz or more), but not by light (0.2 Hz) stimulation. This contrasts with the inhibition at light stimulation induced by perturbation of amphiphysin (Shupliakov et al., 1997). Inhibition by syndapin antibodies was associated with massive accumulation of membranous cisternae and invaginations around release sites, but not of coated pits at the plasma membrane. Cisternae contained vesicle membrane, as shown by vesicle-associated membrane protein 2 (VAMP2)/synaptobrevin 2 immunolabeling. Similar effects were observed when syndapin was perturbed before onset of massive endocytosis induced by preceding intense stimulation. Selective perturbation of the Src homology 3 domain interactions of syndapin was sufficient to induce vesicle depletion and accumulation of cisternae. Our data show an involvement of syndapin in synaptic vesicle recycling evoked by intense stimulation. We propose that syndapin is required to stabilize the plasma membrane and/or facilitate bulk endocytosis at high release rates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.