ArticleJournal of virology2008
Systematic study of the functions for the residues around the nucleotide pocket in simian virus 40 AAA+ hexameric helicase.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Molecular Characterization of Helicase and Nuclease Domains in PPV5 NS1 from Mexican Full-Length Sequence.Viruses · 2026Article
- Molecular switch-like regulation enables global subunit coordination in a viral ring ATPase.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Study of SV40 large T antigen nucleotide specificity for DNA unwinding.Virology journal · 2017Article
- Article
- The conserved core enzymatic activities and the distinct dynamics of polyomavirus large T antigens.Archives of biochemistry and biophysics · 2015Article
- Mechanism of origin DNA recognition and assembly of an initiator-helicase complex by SV40 large tumor antigen.Cell reports · 2013Article
- Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7.BMC biochemistry · 2013Article
- The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.Microbiology and molecular biology reviews : MMBR · 2012Review
- ATPase site architecture is required for self-assembly and remodeling activity of a hexameric AAA+ transcriptional activator.Molecular cell · 2012Article
- Conserved motifs involved in ATP hydrolysis by MalT, a signal transduction ATPase with numerous domains from Escherichia coli.Journal of bacteriology · 2010Article
- Mutational analysis of an archaeal minichromosome maintenance protein exterior hairpin reveals critical residues for helicase activity and DNA binding.BMC molecular biology · 2010Article
- Insights into the MCM functional mechanism: lessons learned from the archaeal MCM complex.Critical reviews in biochemistry and molecular biology · 2010Review
- Engineered interfaces of an AAA+ ATPase reveal a new nucleotide-dependent coordination mechanism.The Journal of biological chemistry · 2010Article
- Effect of ATP binding and hydrolysis on dynamics of canine parvovirus NS1.Journal of virology · 2010Article
- 3.3 A cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry.Cell · 2010Article
- A computational analysis of ATP binding of SV40 large tumor antigen helicase motor.PLoS computational biology · 2009Article
- Simulating the electrostatic guidance of the vectorial translocations in hexameric helicases and translocases.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Validation of BKV large T-antigen ATP-binding site as a target for drug discovery.Antiviral research · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The high-resolution structural data for simian virus 40 large-T-antigen helicase revealed a set of nine residues bound to ATP/ADP directly or indirectly. The functional role of each of these residues in ATP hydrolysis and also the helicase function of this AAA+ (ATPases associated with various cellular activities) molecular motor are unclear. Here, we report our mutational analysis of each of these residues to examine their functionality in oligomerization, DNA binding, ATP hydrolysis, and double-stranded DNA (dsDNA) unwinding. All mutants were capable of oligomerization in the presence of ATP and could bind single-stranded DNA and dsDNA. ATP hydrolysis was substantially reduced for proteins with mutations of residues making direct contact with the gamma-phosphate of ATP or the apical water molecule. A potentially noncanonical "arginine finger" residue, K418, is critical for ATP hydrolysis and helicase function, suggesting a new type of arginine finger role by a lysine in the stabilization of the transition state during ATP hydrolysis. Interestingly, our mutational data suggest that the positive- and negative-charge interactions in the uniquely observed residue pairs, R498/D499 and R540/D502, in large-T-antigen helicase are critically involved in the transfer of energy of ATP binding/hydrolysis to DNA unwinding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.