Evidence map›Paper›PMID 18400796›Full record

ArticleClinical microbiology reviews2008

Molecular genetic basis of ribotyping.

Valérie Bouchet, Heather Huot, Richard Goldstein

Open access · greenAbstract read
In one paragraph

Article in Clinical microbiology reviews, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 96 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Comparison of Automated Ribotyping,International journal of molecular sciences · 2022
    Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Methods for Studying Gut Microbiota: A Primer for Physicians.Journal of clinical and experimental hepatology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Valérie BouchetSection of Molecular Genetics, Division of Pediatric Infectious Diseases, The Maxwell Finland Laboratory for Infectious Diseases, Boston University School of Medicine, Boston Medical Center, BioSquare-III, Boston, MA 02118, USA.
Heather Huot
Richard Goldstein
Boston University · USBoston Medical Center · US

Funding

VACCINATION AND THE EVOLUTIONARY DYNAMICS OF PNEUMOCOCCIR01AI048935 · NIAID · HARVARD UNIVERSITY (SCH OF PUBLIC HLTH) · PI LIPSITCH, MARC · 2001 to 2016
$8.8M
NTHi LPS: virulence factor and vaccine candidateR01DC005855 · NIDCD · BOSTON UNIVERSITY MEDICAL CAMPUS · PI GOLDSTEIN, RICHARD · 2003 to 2006
$1.5M
Role of sialylated LPS in H. influenzae otitis mediaR21DC005564 · NIDCD · BOSTON MEDICAL CENTER · PI GOLDSTEIN, RICHARD · 2002 to 2003
$161k
MICROBIAL POPULATION STRATEGY FOR VACCINE DEVELOPMENTR55DC004583 · NIDCD · BOSTON MEDICAL CENTER · PI GOLDSTEIN, RICHARD · 2001 to 2001
$100k
NIAID NIH HHS AI048935NIAID NIH HHS R01 AI048935NIDCD NIH HHS DC005855NIDCD NIH HHS DC04583NIDCD NIH HHS DC05564NIDCD NIH HHS R01 DC005855NIDCD NIH HHS R21 DC005564NIDCD NIH HHS R55 DC004583
6 · The paper itself

Abstract

Nearly 2,000 ribotyping-based studies exist, ranging from epidemiology to phylogeny and taxonomy. None precisely reveals the molecular genetic basis, with many incorrectly attributing detected polymorphisms to rRNA gene sequences. Based on in silico genomics, we demonstrate that ribotype polymorphisms result from sequence variability in neutral housekeeping genes flanking rRNA operons, with rRNA gene sequences serving solely as conserved, flank-linked tags. We also reveal that from such an informatics perspective, it is readily feasible a priori to design an interpretable ribotyping scheme for a genomically sequenced microbial species, and we discuss limitations to the basic restriction fragment length polymorphism-based method as well as alternate PCR ribotyping-based schemes.

Indexed as

rRNA OperonBacterial Typing TechniquesDNA, BacterialDNA, RibosomalGenes, rRNAMolecular BiologyPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthRibotypingDNA, BacterialDNA, Ribosomal

Identifiers

PMID18400796
PMCPMC2292578
OpenAlexW2125039550

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.