ArticleThe Journal of biological chemistry2008
Interactions between the yeast SM22 homologue Scp1 and actin demonstrate the importance of actin bundling in endocytosis.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 42 citations in OpenAlex.
- A role for cross-linking proteins in actin filament network organization and force generation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A Role for Cross-linking Proteins in Actin Filament Network Organization and Force Generation.bioRxiv : the preprint server for biology · 2024Article
- Proteomic Markers for Mechanobiological Properties of Metastatic Cancer Cells.International journal of molecular sciences · 2023Review
- Red CdSe/ZnS QDs' Intracellular Trafficking and Its Impact on Yeast Polarization and Actin Filament.Cells · 2023Article
- Transgelin: a new gene involved in LDL endocytosis identified by a genome-wide CRISPR-Cas9 screen.Journal of lipid research · 2022Article
- Plastin 3 in health and disease: a matter of balance.Cellular and molecular life sciences : CMLS · 2021Review
- Diversification of the calponin family proteins by gene amplification and repeat expansion of calponin-like motifs.Cytoskeleton (Hoboken, N.J.) · 2021Article
- Novel Mouse Model Reveals That Serine Phosphorylation of L-Plastin Is Essential for Effective Splenic Clearance of Pneumococcus.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- TwoThe Journal of biological chemistry · 2020Article
- Mechanical stiffness of reconstituted actin patches correlates tightly with endocytosis efficiency.PLoS biology · 2019Article
- The contributions of the actin machinery to endocytic membrane bending and vesicle formation.Molecular biology of the cell · 2018Article
- The Impact of ESCRT on AβFrontiers in molecular neuroscience · 2018Article
- Fscn1 is required for the trafficking of TGF-β family type I receptors during endoderm formation.Nature communications · 2016Article
- Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Distinct Actin and Lipid Binding Sites in Ysc84 Are Required during Early Stages of Yeast Endocytosis.PloS one · 2015Article
- Live cell imaging of membrane/cytoskeleton interactions and membrane topology.Scientific reports · 2014Article
- Role of turgor pressure in endocytosis in fission yeast.Molecular biology of the cell · 2014Article
- An Abp1-dependent route of endocytosis functions when the classical endocytic pathway in yeast is inhibited.PloS one · 2014Article
- Role of actin-bundling protein Sac6 in growth of Cryptococcus neoformans at low oxygen concentration.Eukaryotic cell · 2012Article
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
The yeast SM22 homologue Scp1 has previously been shown to act as an actin-bundling protein in vitro. In cells, Scp1 localizes to the cortical actin patches that form as part of the invagination process during endocytosis, and its function overlaps with that of the well characterized yeast fimbrin homologue Sac6p. In this work we have used live cell imaging to demonstrate the importance of key residues in the Scp1 actin interface. We have defined two actin binding domains within Scp1 that allow the protein to both bind and bundle actin without the need for dimerization. Green fluorescent protein-tagged mutants of Scp1 also indicate that actin localization does not require the putative phosphorylation site Ser-185 to be functional. Deletion of SCP1 has few discernable effects on cell growth and morphology. However, we reveal that scp1 deletion is compensated for by up-regulation of Sac6. Furthermore, Scp1 levels are increased in the absence of sac6. The presence of compensatory pathways to up-regulate Sac6 or Scp1 levels in the absence of the other suggest that maintenance of sufficient bundling activity is critical within the cell. Analysis of cortical patch assembly and movement during endocytosis reveals a previously undetected role for Scp1 in movement of patches away from the plasma membrane. Additionally, we observe a dramatic increase in patch lifetime in a strain lacking both sac6 and scp1, demonstrating the central role played by actin-bundling proteins in the endocytic process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.