ArticleThe Journal of biological chemistry2008
Histone acetylation and methylation at sites initiating divergent polycistronic transcription in Trypanosoma cruzi.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 106 citations in OpenAlex.
- Transcription Start Regions in PTU-intergenic regions drive cell cycle-dependent transcriptional activation events inMicrobiology spectrum · 2026Article
- Determining Histone Posttranslational Modifications Along the Cell Cycle in Trypanosoma cruzi by Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Histone post-translational modifications of Leishmania braziliensis.Scientific data · 2025Article
- Trypanosomatid histones: the building blocks of the epigenetic code of highly divergent eukaryotes.The Biochemical journal · 2025Review
- Histone H3 K4 trimethylation occurs mainly at the origins of polycistronic transcription in the genome of Leishmania infantum promastigotes and intracellular amastigotes.BMC genomics · 2025Article
- Mono-allelic epigenetic regulation of polycistronic transcription initiation by RNA polymerase II inmBio · 2025Article
- Protein phosphatase PP1 regulation of RNA polymerase II transcription termination and allelic exclusion of VSG genes in trypanosomes.Nucleic acids research · 2024Article
- Leishmania PNUTS discriminates between PP1 catalytic subunits through an RVxF-ΦΦ-F motif and polymorphisms in the PP1 C-tail and catalytic domain.The Journal of biological chemistry · 2023Article
- Genome-wide chromatin interaction map for Trypanosoma cruzi.Nature microbiology · 2023Article
- Knockout of protein phosphatase 1 in Leishmania major reveals its role during RNA polymerase II transcription termination.Nucleic acids research · 2023Article
- Open chromatin analysis in Trypanosoma cruzi life forms highlights critical differences in genomic compartments and developmental regulation at tDNA loci.Epigenetics & chromatin · 2022Article
- Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4-deficient Trypanosoma cruzi cells.Scientific reports · 2021Article
- Protein acetylation in the critical biological processes in protozoan parasites.Trends in parasitology · 2021Review
- An updated map of Trypanosoma cruzi histone post-translational modifications.Scientific data · 2021Article
- Nucleosome landscape reflects phenotypic differences in Trypanosoma cruzi life forms.PLoS pathogens · 2021Article
- Review
- The Experimental Proteome ofGenes · 2020Article
- Review
- Characterising ISWI chromatin remodeler in Trypanosoma cruzi.Memorias do Instituto Oswaldo Cruz · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypanosomes are ancient eukaryotic parasites in which the protein-coding genes, organized in large polycistronic clusters on both strands, are transcribed from as yet unidentified promoters. In an effort to reveal transcriptional initiation sites, we examined the Trypanosoma cruzi genome for histone modification patterns shown to be linked to active genes in various organisms. Here, we show that acetylated and methylated histones were found to be enriched at strand switch regions of divergent gene arrays, not at convergent clusters or intra- and intergenic regions within clusters. The modified region showed a bimodular profile with two peaks centered over the 5'-regions of the gene pair flanking the strand switch region. This pattern, which demarcates polycistronic transcription units originating from bidirectional initiation sites, is likely to be common in kinetoplastid parasites as well as in other organisms with polycistronic transcription. In contrast, no acetylation was found at promoters of the highly expressed rRNA and spliced leader genes or satellite DNA or at tested retrotransposonal elements. These results reveal, for the first time, the presence of specific epigenetic marks in T. cruzi with potential implications for transcriptional regulation; they indicate that both histone modifications and bidirectional transcription are evolutionarily conserved.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.