ArticleStructure (London, England : 1993)2008
Structures of the rare-cutting restriction endonuclease NotI reveal a unique metal binding fold involved in DNA binding.
Article in Structure (London, England : 1993), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
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- A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression.BMC biotechnology · 2020Article
- An Iron Reservoir to the Catalytic Metal: THE RUBREDOXIN IRON IN AN EXTRADIOL DIOXYGENASE.The Journal of biological chemistry · 2015Article
- Analysis of the HindIII-catalyzed reaction by time-resolved crystallography.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Type II restriction endonucleases--a historical perspective and more.Nucleic acids research · 2014Review
- The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity.Nucleic acids research · 2014Article
- Rational engineering of sequence specificity in R.MwoI restriction endonuclease.Nucleic acids research · 2012Article
- Sequence, structure and functional diversity of PD-(D/E)XK phosphodiesterase superfamily.Nucleic acids research · 2012Article
- Re-visiting protein-centric two-tier classification of existing DNA-protein complexes.BMC bioinformatics · 2012Article
- Recognition of an expanded genetic alphabet by type-II restriction endonucleases and their application to analyze polymerase fidelity.Nucleic acids research · 2011Article
- DNA intercalation without flipping in the specific ThaI-DNA complex.Nucleic acids research · 2011Article
- Folding, DNA recognition, and function of GIY-YIG endonucleases: crystal structures of R.Eco29kI.Structure (London, England : 1993) · 2010Article
- Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA.Protein expression and purification · 2010Article
- DNA-mediated charge transport in redox sensing and signaling.Journal of the American Chemical Society · 2010Article
- Structural mechanisms for the 5'-CCWGG sequence recognition by the N- and C-terminal domains of EcoRII.Nucleic acids research · 2009Article
- An iron-sulfur cluster is essential for the binding of broken DNA by AddAB-type helicase-nucleases.The Journal of biological chemistry · 2009Article
- The structure of SgrAI bound to DNA; recognition of an 8 base pair target.Nucleic acids research · 2008Article
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8 authors at 3 institutions in 1 country.
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Abstract
The structure of the rare-cutting restriction endonuclease NotI, which recognizes the 8 bp target 5'-GCGGCCGC-3', has been solved with and without bound DNA. Because of its specificity (recognizing a site that occurs once per 65 kb), NotI is used to generate large genomic fragments and to map DNA methylation status. NotI contains a unique metal binding fold, found in a variety of putative endonucleases, occupied by an iron atom coordinated within a tetrahedral Cys4 motif. This domain positions nearby protein elements for DNA recognition, and serves a structural role. While recognition of the central six base pairs of the target is accomplished via a saturated hydrogen bond network typical of restriction enzymes, the most peripheral base pairs are engaged in a single direct contact in the major groove, reflecting reduced pressure to recognize those positions. NotI may represent an evolutionary intermediate between mobile endonucleases (which recognize longer target sites) and canonical restriction endonucleases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.