ArticlePLoS biology2008
Darwinian evolution on a chip.
Article in PLoS biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Evolution at the Origins of Life?Life (Basel, Switzerland) · 2024Review
- Automated in vitro evolution of a translation-coupled RNA replication system in a droplet flow reactor.Scientific reports · 2018Article
- In vivo continuous directed evolution.Current opinion in chemical biology · 2015Review
- Limits of neutral drift: lessons from the in vitro evolution of two ribozymes.Journal of molecular evolution · 2014Article
- Continuous evolution: protein evolution at warp speed.Nature chemical biology · 2011Article
- Microfluidic landscapes for evolution.Current opinion in chemical biology · 2010Review
- Image-based feedback control for real-time sorting of microspheres in a microfluidic device.Lab on a chip · 2010Article
- Microfluidic compartmentalized directed evolution.Chemistry & biology · 2010Article
- Niche partitioning in the coevolution of 2 distinct RNA enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Evolution in an RNA world.Cold Spring Harbor symposia on quantitative biology · 2009Review
- Array-based evolution of DNA aptamers allows modelling of an explicit sequence-fitness landscape.Nucleic acids research · 2009Article
- Man versus machine versus ribozyme.PLoS biology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Computer control of Darwinian evolution has been demonstrated by propagating a population of RNA enzymes in a microfluidic device. The RNA population was challenged to catalyze the ligation of an oligonucleotide substrate under conditions of progressively lower substrate concentrations. A microchip-based serial dilution circuit automated an exponential growth phase followed by a 10-fold dilution, which was repeated for 500 log-growth iterations. Evolution was observed in real time as the population adapted and achieved progressively faster growth rates over time. The final evolved enzyme contained a set of 11 mutations that conferred a 90-fold improvement in substrate utilization, coinciding with the applied selective pressure. This system reduces evolution to a microfluidic algorithm, allowing the experimenter to observe and manipulate adaptation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.