ArticleBrain cell biology2006
Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon.
Article in Brain cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 93 citations in OpenAlex.
- Prolonged Hyperactivity Elicits Massive and Persistent Chloride Ion Redistribution in Subsets of Cultured Hippocampal Dentate Granule Cells.bioRxiv : the preprint server for biology · 2025Article
- Murine Retina Outer Plexiform Layer Development and Transcriptome Analysis of Pre-Synapses in Photoreceptors.Life (Basel, Switzerland) · 2024Article
- Synthesis and Characterization of a Novel Concentration-Independent Fluorescent Chloride Indicator, ABP-Dextran, Optimized for Extracellular Chloride Measurement.Biomolecules · 2024Article
- Correlations of receptor desensitization of gain-of-function GABRB3 variants with clinical severity.Brain : a journal of neurology · 2024Article
- Developmental changes in brain activity of heterozygousFrontiers in neurology · 2023Article
- Observing and Quantifying Fluorescent Reporters.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Unique Actions of GABA Arising from Cytoplasmic Chloride Microdomains.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Intracellular ClPflugers Archiv : European journal of physiology · 2020Review
- Increased intracellular ClTheranostics · 2020Article
- Biophysical Modeling Suggests Optimal Drug Combinations for Improving the Efficacy of GABA Agonists after Traumatic Brain Injuries.Journal of neurotrauma · 2019Article
- Bumetanide Prevents Brain Trauma-Induced Depressive-Like Behavior.Frontiers in molecular neuroscience · 2019Article
- Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes.Nature communications · 2018Article
- GABANature communications · 2017Article
- Simultaneous two-photon imaging of intracellular chloride concentration and pH in mouse pyramidal neurons in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A noninvasive optical approach for assessing chloride extrusion activity of the K-Cl cotransporter KCC2 in neuronal cells.BMC neuroscience · 2017Article
- Dysbindin Deficiency Modifies the Expression of GABA Neuron and Ion Permeation Transcripts in the Developing Hippocampus.Frontiers in genetics · 2017Article
- A simple approach for measuring FRET in fluorescent biosensors using two-photon microscopy.Nature protocols · 2016Article
- Article
- Spontaneous Release Regulates Synaptic Scaling in the Embryonic Spinal Network In Vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Optogenetic Visualization of Presynaptic Tonic Inhibition of Cerebellar Parallel Fibers.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
We describe here a molecular genetic approach for imaging synaptic inhibition. The thy-1 promoter was used to express high levels of Clomeleon, a ratiometric fluorescent indicator for chloride ions, in discrete populations of neurons in the brains of transgenic mice. Clomeleon was functional after chronic expression and provided non-invasive readouts of intracellular chloride concentration ([Cl(-)](i)) in brain slices, allowing us to quantify age-dependent declines in resting [Cl(-)](i) during neuronal development. Activation of hippocampal interneurons caused [Cl(-)](i) to rise transiently in individual postsynaptic pyramidal neurons. [Cl(-)](i) increased in direct proportion to the amount of inhibitory transmission, with peak changes as large as 4 mM. Integrating responses over populations of pyramidal neurons allowed sensitive detection of synaptic inhibition. Thus, Clomeleon imaging permits non-invasive, spatiotemporally resolved recordings of [Cl(-)](i) in a large variety of neurons, opening up new opportunities for imaging synaptic inhibition and other forms of chloride signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.