ArticlePloS one2008
Inactivation of [Fe-S] metalloproteins mediates nitric oxide-dependent killing of Burkholderia mallei.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- The therapeutic potential of high-dose inhaled nitric oxide for antimicrobial effects: a narrative review and future directions.Intensive care medicine experimental · 2026Review
- Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis-A Comparative Study.Animals : an open access journal from MDPI · 2025Article
- Evaluation of the Diagnostic Accuracy of Exhaled Nitric Oxide as a Marker of Infection and Sepsis in Emergency Department Patients.Emergency medicine international · 2025Article
- Fe-S cluster homeostasis and beyond: The multifaceted roles of IscR.Biochimica et biophysica acta. Molecular cell research · 2024Review
- The nitric oxide paradox: antimicrobial and inhibitor of antibiotic efficacy.Emerging topics in life sciences · 2024Article
- Detection of nitric oxide-mediated metabolic effects using real-time extracellular flux analysis.PloS one · 2024Article
- Local delivery of gaseous signaling molecules for orthopedic disease therapy.Journal of nanobiotechnology · 2023Review
- Article
- Review
- Nitric Oxide Inhibition of Rickettsia rickettsii.Infection and immunity · 2021Article
- Article
- Identification of a PadR-type regulator essential for intracellular pathogenesis of Burkholderia pseudomallei.Scientific reports · 2021Article
- Porcine Alveolar Macrophages' Nitric Oxide Synthase-Mediated Generation of Nitric Oxide Exerts Important Defensive Effects againstPathogens (Basel, Switzerland) · 2019Article
- Plasma-sensitive Escherichia coli mutants reveal plasma resistance mechanisms.Journal of the Royal Society, Interface · 2019Article
- Zinc-dependent substrate-level phosphorylation powers Salmonella growth under nitrosative stress of the innate host response.PLoS pathogens · 2018Article
- Symmetrical (SDMA) and asymmetrical dimethylarginine (ADMA) in sepsis: high plasma levels as combined risk markers for sepsis survival.Critical care (London, England) · 2018Article
- Relative susceptibility of airway organisms to antimicrobial effects of nitric oxide.International forum of allergy & rhinology · 2017Article
- Markers of nitric oxide are associated with sepsis severity: an observational study.Critical care (London, England) · 2017Observational
- HAL2 overexpression induces iron acquisition in bdf1Δ cells and enhances their salt resistance.Current genetics · 2017Article
- Bacterial iron-sulfur cluster sensors in mammalian pathogens.Metallomics : integrated biometal science · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMuch remains to be known about the mechanisms by which O(2)-dependent host defenses mediate broad antimicrobial activity. METHODOLOGY/PRINCIPAL
findingsWe show herein that reactive nitrogen species (RNS) generated by inducible nitric oxide (NO) synthase (iNOS) account for the anti-Burkholderia mallei activity of IFNgamma-primed macrophages. Inducible NOS-mediated intracellular killing may represent direct bactericidal activity, because B. mallei showed an exquisite sensitivity to NO generated chemically. Exposure of B. mallei to sublethal concentrations of NO upregulated transcription of [Fe-S] cluster repair genes, while damaging the enzymatic activity of the [Fe-S] protein aconitase. To test whether [Fe-S] clusters are critical targets for RNS-dependent killing of B. mallei, a mutation was constructed in the NO-induced, [Fe-S] cluster repair regulator iscR. Not only was the iscR mutant hypersusceptible to iNOS-mediated killing, but its aconitase pool was readily oxidized by NO donors as compared to wild-type controls. Although killed by authentic H(2)O(2), which also oxidizes [Fe-S] clusters, B. mallei appear to be resilient to NADPH oxidase-mediated cytotoxicity. The poor respiratory burst elicited by this bacterium likely explains why the NADPH oxidase is nonessential to the killing of B. mallei while it is still confined within phagosomes. CONCLUSIONS/SIGNIFICANCE: Collectively, these findings have revealed a disparate role for NADPH oxidase and iNOS in the innate macrophage response against the strict aerobe B. mallei. To the best of our knowledge, this is the first instance in which disruption of [Fe-S] clusters is demonstrated as cause of the bactericidal activity of NO congeners.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.