ArticleJournal of cell science2008
Caveolin-1 alters Ca(2+) signal duration through specific interaction with the G alpha q family of G proteins.
Article in Journal of cell science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Caveolin-1 Regulates Parathyroid Hormone (PTH)-Related Protein (PTHrP) Actions on PTH Receptor Type 1 in Bone Cells.Journal of cellular physiology · 2025Article
- SNX19 Interacts with Caveolin-1 and Flotillin-1 to Regulate DBiomedicines · 2025Article
- Deformation of caveolae impacts global transcription and translation processes through relocalization of cavin-1.The Journal of biological chemistry · 2022Article
- Glucagon's Metabolic Action in Health and Disease.Comprehensive Physiology · 2021Article
- Emerging Role of Caveolin-1 in GLP-1 Action.Frontiers in endocrinology · 2021Review
- Caveolin1 Tyrosine-14 Phosphorylation: Role in Cellular Responsiveness to Mechanical Cues.The Journal of membrane biology · 2020Review
- Regulation of bifunctional proteins in cells: Lessons from the phospholipase Cβ/G protein pathway.Protein science : a publication of the Protein Society · 2020Review
- Mechanical Stretch Redefines Membrane Gαq-Calcium Signaling Complexes.The Journal of membrane biology · 2019Article
- Super-resolution Visualization of Caveola Deformation in Response to Osmotic Stress.The Journal of biological chemistry · 2017Article
- Phospholipase Cβ1 induces membrane tubulation and is involved in caveolae formation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Caveolin-1 regulates osteoclast differentiation by suppressing cFms degradation.Experimental & molecular medicine · 2015Article
- Minireview: Spatial Programming of G Protein-Coupled Receptor Activity: Decoding Signaling in Health and Disease.Molecular endocrinology (Baltimore, Md.) · 2015Review
- Osmotic Stress Reduces Ca2+ Signals through Deformation of Caveolae.The Journal of biological chemistry · 2015Article
- Caveolin-1 alters the pattern of cytoplasmic Ca2+ oscillations and Ca2+-dependent gene expression by enhancing leukotriene receptor desensitization.The Journal of biological chemistry · 2014Article
- A new non-canonical pathway of Gα(q) protein regulating mitochondrial dynamics and bioenergetics.Cellular signalling · 2014Article
- Striatins contain a noncanonical coiled coil that binds protein phosphatase 2A A subunit to form a 2:2 heterotetrameric core of striatin-interacting phosphatase and kinase (STRIPAK) complex.The Journal of biological chemistry · 2014Article
- Discrepancy between fluorescence correlation spectroscopy and fluorescence recovery after photobleaching diffusion measurements of G-protein-coupled receptors.Analytical biochemistry · 2013Article
- Differential regulation of muscarinic M2 and M3 receptor signaling in gastrointestinal smooth muscle by caveolin-1.American journal of physiology. Cell physiology · 2013Article
- A role for G-proteins in directing G-protein-coupled receptor-caveolae localization.Biochemistry · 2012Article
- Measuring fast calcium fluxes in cardiomyocytes.Journal of visualized experiments : JoVE · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Caveolae are membrane domains having caveolin-1 (Cav1) as their main structural component. Here, we determined whether Cav1 affects Ca(2+) signaling through the Galpha(q)-phospholipase-Cbeta (PLCbeta) pathway using Fischer rat thyroid cells that lack Cav1 (FRTcav(-)) and a sister line that forms caveolae-like domains due to stable transfection with Cav1 (FRTcav(+)). In the resting state, we found that eCFP-Gbetagamma and Galpha(q)-eYFP are similarly associated in both cell lines by Forster resonance energy transfer (FRET). Upon stimulation, the amount of FRET between Galpha(q)-eYFP and eCFP-Gbetagamma remains high in FRTcav(-) cells, but decreases almost completely in FRTcav(+) cells, suggesting that Cav1 is increasing the separation between Galpha(q)-Gbetagamma subunits. In FRTcav(-) cells overexpressing PLCbeta, a rapid recovery of Ca(2+) is observed after stimulation. However, FRTcav(+) cells show a sustained level of elevated Ca(2+). FRET and colocalization show specific interactions between Galpha(q) and Cav1 that increase upon stimulation. Fluorescence correlation spectroscopy studies show that the mobility of Galpha(q)-eGFP is unaffected by activation in either cell type. The mobility of eGFP-Gbetagamma remains slow in FRTcav(-) cells but increases in FRTcav(+) cells. Together, our data suggest that, upon stimulation, Galpha(q)(GTP) switches from having strong interactions with Gbetagamma to Cav1, thereby releasing Gbetagamma. This prolongs the recombination time for the heterotrimer, thus causing a sustained Ca(2+) signal.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.