ArticleBrain research2008
Development of ascorbate transporters in brain cortical capillary endothelial cells in culture.
Article in Brain research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Sodium ascorbate versus ascorbic acid in sepsis: a narrative review of emerging cardiovascular and neurological benefits.Critical care (London, England) · 2026Review
- Unwinding the potentials of vitamin C in COVID-19 and other diseases: An updated review.Nutrition and health · 2023Review
- High-Dose Vitamin C Prevents Secondary Brain Damage After Stroke via Epigenetic Reprogramming of Neuroprotective Genes.Translational stroke research · 2022Article
- A Triple Combination of Targeting Ligands Increases the Penetration of Nanoparticles across a Blood-Brain Barrier Culture Model.Pharmaceutics · 2021Article
- Sodium-dependent vitamin C transporter-2 mediates vitamin C transport at the cortical nerve terminal.Journal of neuroscience research · 2015Article
- Vitamin C in Health and Disease: Its Role in the Metabolism of Cells and Redox State in the Brain.Frontiers in physiology · 2015Review
- Vitamin C Transporters, Recycling and the Bystander Effect in the Nervous System: SVCT2 versus Gluts.Journal of stem cell research & therapy · 2014Article
- Review
- Ascorbate reverses high glucose- and RAGE-induced leak of the endothelial permeability barrier.Biochemical and biophysical research communications · 2014Article
- The nexus of vitamin homeostasis and DNA synthesis and modification in mammalian brain.Molecular brain · 2014Review
- Role of vitamin C in the function of the vascular endothelium.Antioxidants & redox signaling · 2013Review
- Regulation of vitamin C homeostasis during deficiency.Nutrients · 2013Review
- Article
- Article
- The SLC23 family of ascorbate transporters: ensuring that you get and keep your daily dose of vitamin C.British journal of pharmacology · 2011Review
- Differential regulation of the ascorbic acid transporter SVCT2 during development and in response to ascorbic acid depletion.Biochemical and biophysical research communications · 2011Article
- Article
- Low vitamin C and increased oxidative stress and cell death in mice that lack the sodium-dependent vitamin C transporter SVCT2.Free radical biology & medicine · 2010Article
- Transfer of ascorbic acid across the vascular endothelium: mechanism and self-regulation.American journal of physiology. Cell physiology · 2009Article
- Oxidized lipoprotein induces the macrophage ascorbate transporter (SVCT2): protection by intracellular ascorbate against oxidant stress and apoptosis.Archives of biochemistry and biophysics · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Ascorbic acid in its reduced form is not transported across the capillary endothelial cell blood-brain barrier. This is thought to be due to absence of the SVCT2, a specific transporter for ascorbate. To assess this directly we prepared primary cultures of mouse cortical microvascular endothelial cells. When still in the capillaries, these cells did not express the SVCT2 protein as assessed by immunocytochemistry and by immunoblotting. However, during several days in culture, they developed SVCT2 expression and showed ascorbate transport rates comparable to those in immortalized endothelial cell lines. SVCT2 expression was inversely proportional to cell density, was enhanced by culture at low physiologic plasma ascorbate concentrations, was inhibited by ascorbate concentrations expected in the brain interstitium, and was stimulated by cobalt ions. Expression of the SVCT2 was associated with ascorbate-dependent maturation and release of type IV collagen by the cells in culture. Although the SVCT2 is induced by culture of cortical capillary endothelial cells, its absence in vivo remains perplexing, given the need for intracellular ascorbate to facilitate type IV collagen maturation and release by endothelial cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.