ArticleMicrobial cell factories2008
Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry.
Article in Microbial cell factories, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Estimating Constraints for Protection Factors from HDX-MS Data.Biophysical journal · 2019Article
- Characterizing Protein Structure, Dynamics and Conformation in Lyophilized Solids.Current pharmaceutical design · 2015Review
- Functional dynamics of hexameric helicase probed by hydrogen exchange and simulation.Biophysical journal · 2014Article
- Mapping the protein-protein interface between a toxin and its cognate antitoxin from the bacterial pathogen Streptococcus pyogenes.Biochemistry · 2011Article
- Two-stage, self-cycling process for the production of bacteriophages.Microbial cell factories · 2010Article
- Protein-peptide affinity determination using an h/d exchange dilution strategy: application to antigen-antibody interactions.Journal of the American Society for Mass Spectrometry · 2010Article
- Investigating alternative acidic proteases for H/D exchange coupled to mass spectrometry: plasmepsin 2 but not plasmepsin 4 is active under quenching conditions.Journal of the American Society for Mass Spectrometry · 2010Article
- HDX-ESI-MS reveals enhanced conformational dynamics of the amyloidogenic protein beta(2)-microglobulin upon release from the MHC-1.Journal of the American Society for Mass Spectrometry · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.
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Registered trials
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