ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2008
Bipolar, anastral spindle development in artificially activated sea urchin eggs.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Temporal and SUMO-specific SUMOylation contribute to the dynamics of Polo-like kinase 1 (PLK1) and spindle integrity during mouse oocyte meiosis.Developmental biology · 2018Article
- Centrosome amplification and chromosomal instability in human and animal parthenogenetic cell lines.Stem cell reviews and reports · 2012Article
- Cortical cytasters: a highly conserved developmental trait of Bilateria with similarities to Ctenophora.EvoDevo · 2011Article
- The centrosome and bipolar spindle assembly: does one have anything to do with the other?Cell cycle (Georgetown, Tex.) · 2011Article
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7 authors.
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Abstract
The mitotic apparatus of the early sea urchin embryo is the archetype example of a centrosome-dominated, large aster spindle organized by means of the centriole of the fertilizing sperm. In this study, we tested the hypothesis that artificially activated sea urchin eggs possess the capacity to assemble the anastral, bipolar spindles present in many acentrosomal systems. Control fertilized Lytechinus pictus embryos and ammonia-activated eggs were immunolabeled for tubulin, centrosomal material, the spindle pole structuring protein NuMA and the mitotic kinesins MKLP1/Kinesin-6, Eg5/Kinesin-5, and KinI/Kinesin-13. Confocal imaging showed that a subset of ammonia-activated eggs contained bipolar "mini-spindles" that were anastral; displayed metaphase and anaphase-like stages; labeled for centrosomal material, NuMA, and the three mitotic kinesins; and were observed in living eggs using polarization optics. These results suggest that spindle structural and motor proteins have the ability to organize bipolar, anastral spindles in sea urchin eggs activated in the absence of the paternal centriole.
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