ArticleVirchows Archiv : an international journal of pathology2008
Validation of tissue microarray technology in squamous cell carcinoma of the esophagus.
Article in Virchows Archiv : an international journal of pathology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 51 citations in OpenAlex.
- Tissue microarray validation in cervical carcinoma studies. A methodological approach.Histology and histopathology · 2025Article
- High expression of cytokeratin CAM5.2 in esophageal squamous cell carcinoma is associated with poor prognosis.Medicine · 2019Article
- Keratin 14-high subpopulation mediates lung cancer metastasis potentially through Gkn1 upregulation.Oncogene · 2019Article
- Tissue microarray is suitable for scientific biomarkers studies in endometrial cancer.Virchows Archiv : an international journal of pathology · 2018Article
- Tissue microarray technique is applicable to bone marrow biopsies of myeloproliferative neoplasms.Histochemistry and cell biology · 2017Article
- Article
- Clinicopathological implications of the expression of hypoxia-related proteins in gastric cancer.International journal of medical sciences · 2013Article
- Tissue microarray construction for salivary gland tumors study.Medicina oral, patologia oral y cirugia bucal · 2013Article
- Tissue microarray-based evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as tumour prognostic marker.International journal of molecular sciences · 2012Article
- Quantitative analysis of p53 expression in human normal and cancer tissue microarray with global normalization method.International journal of clinical and experimental pathology · 2011Article
- "Analysis of immunohistochemical expression of epidermal growth factor receptor in oral squamous cell carcinoma using tissue microarray technology and whole sections:" A comparative study.Journal of oral and maxillofacial pathology : JOMFPArticle
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue microarray (TMA) technology has been developed to facilitate high-throughput immunohistochemical and in situ hybridization analysis of tissues by inserting small tissue biopsy cores into a single paraffin block. Several studies have revealed novel prognostic biomarkers in esophageal squamous cell carcinoma (ESCC) by means of TMA technology, although this technique has not yet been validated for these tumors. Because representativeness of the donor tissue cores may be a disadvantage compared to full sections, the aim of this study was to assess if TMA technology provides representative immunohistochemical results in ESCC. A TMA was constructed containing triplicate cores of 108 formalin-fixed, paraffin-embedded squamous cell carcinomas of the esophagus. The agreement in the differentiation grade and immunohistochemical staining scores of CK5/6, CK14, E-cadherin, Ki-67, and p53 between TMA cores and a subset of 64 randomly selected donor paraffin blocks was determined using kappa statistics. The concurrence between TMA cores and donor blocks was moderate for Ki-67 (kappa = 0.42) and E-cadherin (kappa = 0.47), substantial for differentiation grade (kappa = 0.65) and CK14 (kappa = 0.71), and almost perfect for p53 (kappa = 0.86) and CK5/6 (kappa = 0.93). TMA technology appears to be a valid method for immunohistochemical analysis of molecular markers in ESCC provided that the staining pattern in the tumor is homogeneous.
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