ArticleProceedings of the National Academy of Sciences of the United States of America2008
Tlx3 exerts context-dependent transcriptional regulation and promotes neuronal differentiation from embryonic stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- From neural crest migration to the onset of gangliogenesis.Current topics in developmental biology · 2025Review
- TLX3 regulates CGN progenitor proliferation during cerebellum development and its dysfunction can lead to autism.iScience · 2024Article
- Tlx3 mediates neuronal differentiation and proper condensation of the developing trigeminal ganglion.Developmental biology · 2024Article
- Molecular Characterization of Nodose Ganglia Development Reveals a Novel Population of Phox2b+ Glial Progenitors in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Promoter hypermethylation of neural-related genes is compatible with stemness in solid cancers.Epigenetics & chromatin · 2023Article
- Epigenetic clock and methylation studies in vervet monkeys.GeroScience · 2022Article
- Glutamatergic Neurons Differentiated from Embryonic Stem Cells: An Investigation of Differentiation and Associated Diseases.International journal of molecular sciences · 2021Review
- Three-Dimensional Otic Neuronal Progenitor Spheroids Derived from Human Embryonic Stem Cells.Tissue engineering. Part A · 2021Article
- Progress in Modeling and Targeting Inner Ear Disorders with Pluripotent Stem Cells.Stem cell reports · 2020Review
- An engineered three-dimensional stem cell niche in the inner ear by applying a nanofibrillar cellulose hydrogel with a sustained-release neurotrophic factor delivery system.Acta biomaterialia · 2020Article
- Vertebrate Sensory Ganglia: Common and Divergent Features of the Transcriptional Programs Generating Their Functional Specialization.Frontiers in cell and developmental biology · 2020Review
- Directed Differentiation of Human Embryonic Stem Cells Toward Placode-Derived Spiral Ganglion-Like Sensory Neurons.Stem cells translational medicine · 2017Article
- Tlx3 promotes glutamatergic neuronal subtype specification through direct interactions with the chromatin modifier CBP.PloS one · 2015Article
- Sound strategies for hearing restoration.Science (New York, N.Y.) · 2014Review
- Dynamics of re-constitution of the human nuclear proteome after cell division is regulated by NLS-adjacent phosphorylation.Cell cycle (Georgetown, Tex.) · 2014Article
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- Reactive oxygen species regulate context-dependent inhibition of NFAT5 target genes.Experimental & molecular medicine · 2013Article
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- Hes-1 regulates the excitatory fate of neural progenitors through modulation of Tlx3 (HOX11L2) expression.Cellular and molecular life sciences : CMLS · 2012Article
- Mutanlallemand (mtl) and Belly Spot and Deafness (bsd) are two new mutations of Lmx1a causing severe cochlear and vestibular defects.PloS one · 2012Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The T cell leukemia 3 (Tlx3) gene has been implicated in specification of glutamatergic sensory neurons in the spinal cord. In cranial sensory ganglia, Tlx3 is highly expressed in differentiating neurons during early embryogenesis. To study a role of Tlx3 during neural differentiation, mouse embryonic stem (ES) cells were transfected with a Tlx3 expression vector. ES cells stably expressing Tlx3 were grown in the presence or absence of a neural induction medium. In undifferentiated ES cells, there was no significant difference in gene expression in the presence or absence of Tlx3, even after ES cells were cultured for an extensive time period. In contrast, expression levels of Mash1, Ngn1, and NeuroD were significantly higher in Tlx3-expressing cells after neural induction for 4 days compared with those in cells expressing the control vector. At 7 days after neural induction, whereas expression of the proneural genes was down-regulated, VGLUT2, GluR2, and GluR4 were significantly increased in ES cell-derived neurons expressing Tlx3. The sequential and coordinated expression of the proneural and neuronal subtype-specific genes identifies Tlx3 as a selector gene in ES cells undergoing neural differentiation. In addition, the differential effects of Tlx3 overexpression in undifferentiated ES cells compared with ES cell-derived neurons suggest that Tlx3 exerts context-dependent transcriptional signals on its downstream target genes. The context-dependent function of Tlx3 as a selector gene may be used to establish a novel strategy to conditionally generate excitatory glutamatergic neurons from ES cells to cure various types of neurodegenerative disorders.
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