ArticleProceedings of the National Academy of Sciences of the United States of America2008
Temperature and pressure dependence of protein stability: the engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Pressure and temperature dependence of fluorescence anisotropy of green fluorescent protein.RSC advances · 2022Article
- General Counteraction Exerted by Sugars against Denaturants.Life (Basel, Switzerland) · 2021Article
- Peptide cargo tunes a network of correlated motions in human leucocyte antigens.The FEBS journal · 2020Article
- Effects of an Electric Field on the Conformational Transition of the Protein: A Molecular Dynamics Simulation Study.Polymers · 2019Article
- Polyubiquitin Drives the Molecular Interactions of the NF-κB Essential Modulator (NEMO) by Allosteric Regulation.The Journal of biological chemistry · 2015Article
- Observation of complete pressure-jump protein refolding in molecular dynamics simulation and experiment.Journal of the American Chemical Society · 2014Article
- Volume of Hsp90 ligand binding and the unfolding phase diagram as a function of pressure and temperature.European biophysics journal : EBJ · 2013Article
- Glycine insertion makes yellow fluorescent protein sensitive to hydrostatic pressure.PloS one · 2013Article
- Effects of pressure and temperature on the binding of RecA protein to single-stranded DNA.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Unique features of the folding landscape of a repeat protein revealed by pressure perturbation.Biophysical journal · 2010Article
- The behavior of the hydrophobic effect under pressure and protein denaturation.Biophysical journal · 2010Article
- Computing the stability diagram of the Trp-cage miniprotein.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Why elliptical stability diagrams are not related to protein conformational disorder.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We have measured the equilibrium constant for the denaturation transition of the engineered fluorescein-binding lipocalin FluA as a function of pressure and temperature, taking advantage of the fact that the ligand's fluorescence is almost fully quenched when complexed with the folded protein, but reversibly reappears on denaturation. From the equilibrium constant as a function of pressure and temperature all of the involved thermodynamic parameters of protein folding, in particular the changes in entropy and volume, compressibility, thermal expansion, and specific heat, were deduced in a global fitting procedure. Assuming that these parameters are independent of temperature and pressure, we can demonstrate from the ratio of Deltabeta, Deltaalpha(2), DeltaC(p) that the phase diagram of protein folding assumes an elliptic shape. Furthermore, we can show that the thermodynamic condition for such an elliptic phase diagram is related to the degree of correlation between the fluctuations of the changes in volume and enthalpy at the phase boundary. For the protein investigated this correlation is low, as generally expected for highly degenerate systems. Our study suggests that the elliptic phase diagram is a consequence of the inherent conformational disorder of proteins and that it may be viewed as the thermodynamic manifestation of the high degeneracy of conformational energies that is characteristic for this class of macromolecules.
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