ArticleMolecular and cellular biology2008
Autoregulation of Npl3, a yeast SR protein, requires a novel downstream region and serine phosphorylation.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- The functionality of telomerase depends on CPF-CF induced 3'end processing of its RNA component TLC1 and a novel Nrd1-Nab3 surveillance mechanism.Nucleic acids research · 2025Article
- The SR-protein Npl3 is an essential component of the meiotic splicing regulatory network in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- The histone variant H2A.Z promotes splicing of weak introns.Genes & development · 2017Article
- Genetic interaction mapping reveals a role for the SWI/SNF nucleosome remodeler in spliceosome activation in fission yeast.PLoS genetics · 2015Article
- Npl3, a new link between RNA-binding proteins and the maintenance of genome integrity.Cell cycle (Georgetown, Tex.) · 2014Article
- The yeast RPL9B gene is regulated by modulation between two modes of transcription termination.The EMBO journal · 2012Article
- Phosphorylation of SRSF1 is modulated by replicational stress.Nucleic acids research · 2012Article
- The yeast SR-like protein Npl3 links chromatin modification to mRNA processing.PLoS genetics · 2012Article
- Keeping mRNPs in check during assembly and nuclear export.Nature reviews. Molecular cell biology · 2011Article
- Molecular mechanisms of eukaryotic pre-mRNA 3' end processing regulation.Nucleic acids research · 2010Review
- Fail-safe transcriptional termination for protein-coding genes in S. cerevisiae.Molecular cell · 2009Article
- Specific sequences within arginine-glycine-rich domains affect mRNA-binding protein function.Nucleic acids research · 2009Article
- Regulation of ARE transcript 3' end processing by the yeast Cth2 mRNA decay factor.The EMBO journal · 2008Article
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3 authors at 1 institution in 1 country.
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Abstract
Npl3 is an SR-like protein with documented roles in mRNA export and transcription termination. Maintaining appropriate levels of Npl3 protein is critical for cell survival. Here we show that Npl3 negatively regulates its own expression via modulation of its mRNA levels. By creating gene chimeras, we demonstrate that the region downstream of the coding sequence of Npl3 is necessary and sufficient to confer regulation. The use of different polyadenylation sites in this region results in at least two stable RNAs; read-through of these sites causes the formation of 3'-extended RNAs that are highly unstable and therefore largely unproductive. Increasing the amount of Npl3 protein promotes read-through. Notably, the loss of Npl3 phosphorylation promotes the use of the productive polyadenylation sites, resulting in elevated levels of Npl3 protein. We propose that proper levels of Npl3 protein are achieved by a negative feedback loop in which phosphorylated Npl3 suppresses efficient recognition of the productive processing signals in its own transcript.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.