ArticleJournal of bacteriology2008
Characterization of a novel methanol dehydrogenase in representatives of Burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 128 citations in OpenAlex.
- Autotrophic methylotrophy with no methanol dehydrogenase (MDH) in a strain of fluorescentPeerJ · 2026Article
- Characterization of endophytic bacteriome diversity and associated beneficial bacteria inhabiting a macrophyteFrontiers in plant science · 2023Article
- The rhizospheric bacterial diversity of Fritillaria taipaiensis under single planting pattern over five years.Scientific reports · 2022Article
- Aerobic Utilization of Methanol for Microbial Growth and Production.Advances in biochemical engineering/biotechnology · 2022Article
- Adaption and Degradation Strategies of Methylotrophic 1,4-Dioxane Degrading StrainInternational journal of molecular sciences · 2021Article
- North Sea spring bloom-associated Gammaproteobacteria fill diverse heterotrophic niches.Environmental microbiome · 2021Article
- Genomic Analysis of Family UBA6911 (Group 18Applied and environmental microbiology · 2021Article
- Metal(loid) speciation and transformation by aerobic methanotrophs.Microbiome · 2021Review
- Article
- Anaerobic microbial methanol conversion in marine sediments.Environmental microbiology · 2021Review
- Methane utilizing plant growth-promoting microbial diversity analysis of flooded paddy ecosystem of India.World journal of microbiology & biotechnology · 2021Article
- Methanol Dehydrogenases as a Key Biocatalysts for Synthetic Methylotrophy.Frontiers in bioengineering and biotechnology · 2021Review
- Lanthanide-Dependent Methanol and Formaldehyde Oxidation inMicroorganisms · 2020Article
- Article
- Novel copper-containing membrane monooxygenases (CuMMOs) encoded by alkane-utilizing Betaproteobacteria.The ISME journal · 2020Article
- Evolution in action: habitat transition from sediment to the pelagial leads to genome streamlining in Methylophilaceae.The ISME journal · 2019Article
- Primary ColonizingApplied and environmental microbiology · 2018Article
- Genomics and Biochemistry of Metabolic Pathways for the CIndian journal of microbiology · 2018Article
- Metals and Methanotrophy.Applied and environmental microbiology · 2018Review
- Lanthanide-Dependent Methanol Dehydrogenases of XoxF4 and XoxF5 Clades Are Differentially Distributed Among Methylotrophic Bacteria and They Reveal Different Biochemical Properties.Frontiers in microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Some members of Burkholderiales are able to grow on methanol but lack the genes (mxaFI) responsible for the well-characterized two-subunit pyrroloquinoline quinone-dependent quinoprotein methanol dehydrogenase that is widespread in methylotrophic Proteobacteria. Here, we characterized novel, mono-subunit enzymes responsible for methanol oxidation in four strains, Methyloversatilis universalis FAM5, Methylibium petroleiphilum PM1, and unclassified Burkholderiales strains RZ18-153 and FAM1. The enzyme from M. universalis FAM5 was partially purified and subjected to matrix-assisted laser desorption ionization-time of fight peptide mass fingerprinting. The resulting peptide spectrum was used to identify a gene candidate in the genome of M. petroleiphilum PM1 (mdh2) predicted to encode a type I alcohol dehydrogenase related to the characterized methanol dehydrogenase large subunits but at less than 35% amino acid identity. Homologs of mdh2 were amplified from M. universalis FAM5 and strains RZ18-153 and FAM1, and mutants lacking mdh2 were generated in three of the organisms. These mutants lost their ability to grow on methanol and ethanol, demonstrating that mdh2 is responsible for oxidation of both substrates. Our findings have implications for environmental detection of methylotrophy and indicate that this ability is widespread beyond populations possessing mxaF, the gene traditionally used as a genetic marker for environmental detection of methanol-oxidizing capability. Our findings also have implications for understanding the evolution of methanol oxidation, suggesting a convergence toward the enzymatic function for methanol oxidation in MxaF and Mdh2-type proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.