ArticleEukaryotic cell2008
The nuclear migration protein NUDF/LIS1 forms a complex with NUDC and BNFA at spindle pole bodies.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
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- The KdmB-EcoA-RpdA-SntB (KERS) chromatin regulatory complex controls development, secondary metabolism and pathogenicity in Aspergillus flavus.Fungal genetics and biology : FG & B · 2023Article
- Study of Humoral Responses againstVaccines · 2019Article
- Multinucleation and Polykaryon Formation is Promoted by the EhPC4 Transcription Factor in Entamoeba histolytica.Scientific reports · 2016Article
- EML4 promotes the loading of NUDC to the spindle for mitotic progression.Cell cycle (Georgetown, Tex.) · 2015Article
- Control of multicellular development by the physically interacting deneddylases DEN1/DenA and COP9 signalosome.PLoS genetics · 2013Article
- Live-cell visualization of intracellular interaction between a nuclear migration protein (hNUDC) and the thrombopoietin receptor (Mpl).PloS one · 2012Article
- Structural features and chaperone activity of the NudC protein family.Journal of molecular biology · 2011Article
- Recruitment of the inhibitor Cand1 to the cullin substrate adaptor site mediates interaction to the neddylation site.Molecular biology of the cell · 2011Article
- Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity.Molecular biology reports · 2010Article
- The mammalian NudC-like genes: a family with functions other than regulating nuclear distribution.Cellular and molecular life sciences : CMLS · 2009Review
- Partitioning the apical domain of the Arabidopsis embryo requires the BOBBER1 NudC domain protein.The Plant cell · 2009Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear migration depends on microtubules, the dynein motor complex, and regulatory components like LIS1 and NUDC. We sought to identify new binding partners of the fungal LIS1 homolog NUDF to clarify its function in dynein regulation. We therefore analyzed the association between NUDF and NUDC in Aspergillus nidulans. NUDF and NUDC directly interacted in yeast two-hybrid experiments via NUDF's WD40 domain. NUDC-green fluorescent protein (NUDC-GFP) was localized to immobile dots in the cytoplasm and at the hyphal cortex, some of which were spindle pole bodies (SPBs). We showed by bimolecular fluorescence complementation microscopy that NUDC directly interacted with NUDF at SPBs at different stages of the cell cycle. Applying tandem affinity purification, we isolated the NUDF-associated protein BNFA (for binding to NUDF). BNFA was dispensable for growth and for nuclear migration. GFP-BNFA fusions localized to SPBs at different stages of the cell cycle. This localization depended on NUDF, since the loss of NUDF resulted in the cytoplasmic accumulation of BNFA. BNFA did not bind to NUDC in a yeast two-hybrid assay. These results show that the conserved NUDF and NUDC proteins play a concerted role at SPBs at different stages of the cell cycle and that NUDF recruits additional proteins specifically to the dynein complex at SPBs.
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