ArticleBiophysical journal2008
Measuring diffusion and binding kinetics by contact area FRAP.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 87 citations in OpenAlex.
- Graph neural network modeling of receptor interaction kinetics from single-molecule imaging data.bioRxiv : the preprint server for biology · 2026Article
- Inferring diffusion, reaction, and exchange parameters from imperfect FRAP.Biophysical journal · 2025Article
- Binding of Soluble Ligands to Membrane Receptors: A Molecular Dynamics Simulation Study.The journal of physical chemistry. B · 2025Article
- Two-dimensional measurements of receptor-ligand interactions.Frontiers in molecular biosciences · 2023Review
- Inferences from FRAP data are model dependent: A subdiffusive analysis.Biophysical journal · 2022Article
- Supported Lipid Bilayers and the Study of Two-Dimensional Binding Kinetics.Frontiers in molecular biosciences · 2022Review
- Single-cell measurements of two-dimensional binding affinity across cell contacts.Biophysical journal · 2021Article
- Bioengineering tools for probing intracellular events in T lymphocytes.WIREs mechanisms of disease · 2021Review
- Relationship of 2D Affinity to T Cell Functional Outcomes.International journal of molecular sciences · 2020Review
- A modeling platform for the lymphatic system.Journal of theoretical biology · 2020Article
- Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells.Frontiers in immunology · 2020Review
- The Influence of Molecular Reach and Diffusivity on the Efficacy of Membrane-Confined Reactions.Biophysical journal · 2019Article
- [The preparation of supported lipid bilayers and its functional study].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2019Article
- Membrane fluctuations and acidosis regulate cooperative binding of 'marker of self' protein CD47 with the macrophage checkpoint receptor SIRPα.Journal of cell science · 2018Article
- Binding equilibrium and kinetics of membrane-anchored receptors and ligands in cell adhesion: Insights from computational model systems and theory.Cell adhesion & migration · 2016Review
- Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Lipid-Bilayer Dynamics Probed by a Carbon Dot-Phospholipid Conjugate.Biophysical journal · 2016Article
- Force and affinity in ligand discrimination by the TCR.Trends in immunology · 2014Review
- Linking 3D and 2D binding kinetics of membrane proteins by multiscale simulations.Protein science : a publication of the Protein Society · 2014Article
- Mathematical modelling and computational study of two-dimensional and three-dimensional dynamics of receptor-ligand interactions in signalling response mechanisms.Journal of mathematical biology · 2014Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The immunological synapse is a stable intercellular structure that specializes in substance and signal transfer from one immune cell to another. Its formation is regulated in part by the diffusion of adhesion and signaling molecules into, and their binding of countermolecules in the contact area. The stability of immunological synapses allows receptor-ligand interactions to approximate chemical equilibrium despite other dynamic aspects. We have developed a mathematical model that describes the coupled reaction-diffusion process in an established immunological synapse. In this study, we extend a previously described contact area fluorescence recovery after photobleaching (FRAP) experiment to test the validity of the model. The receptor binding activity and lateral mobility of fluorescently labeled, lipid-anchored ligands in the bilayer resulted in their accumulation, as revealed by a much higher fluorescence intensity inside the contact area than outside. After complete photobleaching of the synapse, fluorescence recovery requires ligands to dissociate and rebind, and to diffuse in and out of the contact area. Such a FRAP time course consequently provides information on reaction and diffusion, which can be extracted by fitting the model solution to the data. Surprisingly, reverse rates in the two-dimensional contact area were at least 100-fold slower than in three-dimensional solution. As previously reported in immunological synapses, a significant nonrecoverable fraction of fluorescence was observed with one of two systems studied, suggesting some ligands either dissociated or diffused much more slowly compared with other ligands in the same synapse. The combined theory and experiment thus provides a new method for in situ measurements of kinetic rates, diffusion coefficients, and nonrecoverable fractions of interacting molecules in immunological synapses and other stable cell-bilayer junctions.
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