ArticleThe Journal of biological chemistry2008
Novel splice variants of rat CaV2.1 that lack much of the synaptic protein interaction site are expressed in neuroendocrine cells.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- CaThe Journal of physiology · 2024Article
- The Interplay Between Splicing of Two Exon Combinations Differentially Affects Membrane Targeting and Function of Human CaFunction (Oxford, England) · 2024Article
- Regulation of Presynaptic Calcium Channels.Advances in neurobiology · 2023Article
- Presynaptic voltage-gated calcium channels in the auditory brainstem.Molecular and cellular neurosciences · 2021Review
- Electrophysiological properties of identified oxytocin and vasopressin neurones.Journal of neuroendocrinology · 2019Review
- A novel region in the CaeLife · 2017Article
- The Physiology, Pathology, and Pharmacology of Voltage-Gated Calcium Channels and Their Future Therapeutic Potential.Pharmacological reviews · 2015Review
- Age-dependent contribution of P/Q- and R-type Ca2+ channels to neuromuscular transmission in lethargic mice.The Journal of pharmacology and experimental therapeutics · 2015Article
- The role of voltage-gated calcium channels in neurotransmitter phenotype specification: Coexpression and functional analysis in Xenopus laevis.The Journal of comparative neurology · 2014Article
- The Ca2+ channel β2 subunit is selectively targeted to the axon terminals of supraoptic neurons.Channels (Austin, Tex.) · 2014Article
- Control of neuronal voltage-gated calcium ion channels from RNA to protein.Trends in neurosciences · 2013Review
- Imaging large cohorts of single ion channels and their activity.Frontiers in endocrinology · 2013Review
- Calretinin regulates Ca2+-dependent inactivation and facilitation of Ca(v)2.1 Ca2+ channels through a direct interaction with the α12.1 subunit.The Journal of biological chemistry · 2012Article
- Calcium channel functions in pain processing.Channels (Austin, Tex.)Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Voltage-gated Ca(2+) channels are responsible for the activation of the Ca(2+) influx that triggers exocytotic secretion. The synaptic protein interaction (synprint) site found in the II-III loop of Ca(V)2.1 and Ca(V)2.2 mediates a physical association with synaptic proteins that may be crucial for fast neurotransmission and axonal targeting. We report here the use of nested PCR to identify two novel splice variants of rat Ca(V)2.1 that lack much of the synprint site. Furthermore, we compare immunofluorescence data derived from antibodies directed against sequences in the Ca(V)2.1 synprint site and carboxyl terminus to show that channel variants lacking a portion of the synprint site are expressed in two types of neuroendocrine cells. Immunofluorescence data also suggest that such variants are properly targeted to neuroendocrine terminals. When expressed in a mammalian cell line, both splice variants yielded Ca(2+) currents, but the variant containing the larger of the two deletions displayed a reduced current density and a marked shift in the voltage dependence of inactivation. These results have important implications for Ca(V)2.1 function and for the mechanisms of Ca(V)2.1 targeting in neurons and neuroendocrine cells.
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