ArticlePlant molecular biology2008
The sweet potato sporamin promoter confers high-level phytase expression and improves organic phosphorus acquisition and tuber yield of transgenic potato.
Article in Plant molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 49 citations in OpenAlex.
- High expression of GUS activities in sweet potato storage roots by sucrose-inducible minimal promoter.Plant cell reports · 2019Article
- Comparative transcriptome and metabolome analyses provide new insights into the molecular mechanisms underlying taproot thickening in Panax notoginseng.BMC plant biology · 2019Article
- Effects of a novel corn-expressed E. coli phytase on digestibility of calcium and phosphorous, growth performance, and bone ash in young growing pigs1.Journal of animal science · 2019Article
- Heterologous Expression of Secreted Bacterial BPP and HAP Phytases in Plants StimulatesFrontiers in plant science · 2018Article
- Extracellular Secretion of Phytase from Transgenic Wheat Roots Allows Utilization of Phytate for Enhanced Phosphorus Uptake.Molecular biotechnology · 2017Article
- Genetically modified phytase crops role in sustainable plant and animal nutrition and ecological development: a review.3 Biotech · 2017Review
- Draft Genome Sequence ofGenome announcements · 2017Article
- Genome-wide identification of pistil-specific genes expressed during fruit set initiation in tomato (Solanum lycopersicum).PloS one · 2017Article
- Temporal and spatial control of gene expression in horticultural crops.Horticulture research · 2014Review
Corrections and comments
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Authors and funding
9 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sweet potato sporamin promoter was used to control the expression in transgenic potato of the E. coli appA gene, which encodes a bifunctional enzyme exhibiting both acid phosphatase and phytase activities. The sporamin promoter was highly active in leaves, stems and different size tubers of transgenic potato, with levels of phytase expression ranging from 3.8 to 7.4% of total soluble proteins. Phytase expression levels in transgenic potato tubers were stable over several cycles of propagation. Field tests showed that tuber size, number and yield increased in transgenic potato. Improved phosphorus (P) acquisition when phytate was provided as a sole P source and enhanced microtuber formation in cultured transgenic potato seedlings when phytate was provided as an additional P source were observed, which may account for the increase in leaf chloroplast accumulation (important for photosynthesis) and tuber yield of field-grown transgenic potato supplemented with organic fertilizers. Animal feeding tests indicated that the potato-produced phytase supplement was as effective as a commercially available microbial phytase in increasing the availability of phytate-P to weanling pigs. This study demonstrates that the sporamin promoter can effectively direct high-level recombinant protein expression in potato tubers. Moreover, overexpression of phytase in transgenic potato not only offers an ideal feed additive for improving phytate-P digestibility in monogastric animals but also improves tuber yield, enhances P acquisition from organic fertilizers, and has a potential for phytoremediation.
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