ArticlePflugers Archiv : European journal of physiology2008
Functional involvement of Annexin-2 in cAMP induced AQP2 trafficking.
Article in Pflugers Archiv : European journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Proteomics and AQP2 regulation.The Journal of physiology · 2024Review
- The intricate role of annexin A2 in kidney: a comprehensive review.Renal failure · 2023Review
- Insight into the Mammalian Aquaporin Interactome.International journal of molecular sciences · 2022Review
- Vasopressin-aquaporin-2 pathway: recent advances in understanding water balance disorders.F1000Research · 2019Review
- Article
- Aquaporin Protein-Protein Interactions.International journal of molecular sciences · 2017Review
- Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5.The Journal of biological chemistry · 2017Article
- Novel Aquaporin Regulatory Mechanisms Revealed by Interactomics.Molecular & cellular proteomics : MCP · 2016Article
- The Trafficking of the Water Channel Aquaporin-2 in Renal Principal Cells-a Potential Target for Pharmacological Intervention in Cardiovascular Diseases.Frontiers in pharmacology · 2016Review
- Annexin A2 regulates TRPA1-dependent nociception.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Expanding the proteome of an RNA virus by phosphorylation of an intrinsically disordered viral protein.The Journal of biological chemistry · 2014Article
- Annexin A2 mediates apical trafficking of renal Na⁺-K⁺-2Cl⁻ cotransporter.The Journal of biological chemistry · 2014Article
- Article
- Cell biology of vasopressin-regulated aquaporin-2 trafficking.Pflugers Archiv : European journal of physiology · 2012Review
- Cell culture models and animal models for studying the patho-physiological role of renal aquaporins.Cellular and molecular life sciences : CMLS · 2012Review
- Aquaporin-2 in the "-omics" era.The Journal of biological chemistry · 2009Review
- Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometry.Journal of proteome research · 2009Article
- Membrane rafts and caveolae in cardiovascular signaling.Current opinion in nephrology and hypertension · 2009Review
- Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct.American journal of physiology. Cell physiology · 2008Article
- Introduction for Special issue for Aquaporin: expanding the world of aquaporins: new members and new functions.Pflugers Archiv : European journal of physiology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Annexin-2 is required for the apical transport in epithelial cells. In this study, we investigated the involvement of annexin-2 in cAMP-induced aquaporin-2 (AQP2) translocation to the apical membrane in renal cells. We found that the cAMP-elevating agent forskolin increased annexin-2 abundance in the plasma membrane enriched fraction with a parallel decrease in the soluble fraction. Interestingly, forskolin stimulation resulted in annexin-2 enrichment in lipid rafts, suggesting that hormonal stimulation might be responsible for a new configuration of membrane interacting proteins involved in the fusion of AQP2 vesicles to the apical plasma membrane. To investigate the functional involvement of annexin-2 in AQP2 exocytosis, the fusion process between purified AQP2 membrane vesicles and plasma membranes was reconstructed in vitro and monitored by a fluorescence assay. An N-terminal peptide that comprises 14 residues of annexin-2 and that includes the binding site for the calcium binding protein p11 strongly inhibited the fusion process. Preincubation of cells with this annexin-2 peptide also failed to increase the osmotic water permeability in the presence of forskolin in intact cells. Altogether, these data demonstrate that annexin-2 is required for cAMP-induced AQP2 exocytosis in renal cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.