ArticleNature protocols2008
Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression.
Article in Nature protocols, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Current strategies and opportunities to manufacture cells for modeling human lungs.Advanced drug delivery reviews · 2020Pooled it
- Leveraging microtopography to pattern multi-oriented muscle actuators.Biomaterials science · 2025Article
- Basal delamination during mouse gastrulation primes pluripotent cells for differentiation.Developmental cell · 2024Article
- Tissue geometry drives deterministic organoid patterning.Science (New York, N.Y.) · 2022Article
- Guided Self-Assembly of ES-Derived Lung Progenitors into Biomimetic Tube Structures That Impact Cell Differentiation.Bioengineering (Basel, Switzerland) · 2021Article
- Dynamic changes in epithelial cell packing during tissue morphogenesis.Current biology : CB · 2021Review
- 3D printing of sacrificial thioester elastomers using digital light processing for templating 3D organoid structures in soft biomatrices.Biofabrication · 2021Article
- Microtissue Geometry and Cell-Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D.Advanced healthcare materials · 2021Article
- Fabrication Method of a High-Density Co-Culture Tumor-Stroma Platform to Study Cancer Progression.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The not-so-sweet side of sugar: Influence of the microenvironment on the processes that unleash cancer.Biochimica et biophysica acta. Molecular basis of disease · 2020Review
- Behaviors of Glioblastoma Cells in in Vitro Microenvironments.Scientific reports · 2019Article
- Breast Cancer Heterogeneity: A focus on Epigenetics and In Vitro 3D Model Systems.Cell journal · 2018Article
- The Role of Desmoplasia and Stromal Fibroblasts on Anti-cancer Drug Resistance in a Microengineered Tumor Model.Cellular and molecular bioengineering · 2018Article
- Opportunities for organoids as new models of aging.The Journal of cell biology · 2018Review
- Engineering 3D approaches to model the dynamic microenvironments of cancer bone metastasis.Bone research · 2018Review
- Growth of hollow cell spheroids in microbead templated chambers.Biomaterials · 2017Article
- Dynamics of Tissue-Induced Alignment of Fibrous Extracellular Matrix.Biophysical journal · 2017Article
- Microfabricated tissues for investigating traction forces involved in cell migration and tissue morphogenesis.Cellular and molecular life sciences : CMLS · 2017Review
- Organotypic culture in three dimensions prevents radiation-induced transformation in human lung epithelial cells.Scientific reports · 2016Article
- 3D matrix-based cell cultures: Automated analysis of tumor cell survival and proliferation.International journal of oncology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Here, we describe a simple micromolding method to construct three-dimensional arrays of organotypic epithelial tissue structures that approximate in vivo histology. An elastomeric stamp containing an array of posts of defined geometry and spacing is used to mold microscale cavities into the surface of type I collagen gels. Epithelial cells are seeded into the cavities and covered with a second layer of collagen. The cells reorganize into hollow tissues corresponding to the geometry of the cavities. Patterned tissue arrays can be produced in 3-4 h and will undergo morphogenesis over the following 1-3 d. The protocol can easily be adapted to study a variety of tissues and aspects of normal and neoplastic development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.