ArticleJournal of cell science2008
Recycling of IRAP from the plasma membrane back to the insulin-responsive compartment requires the Q-SNARE syntaxin 6 but not the GGA clathrin adaptors.
Article in Journal of cell science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- GLUT4 Trafficking and Storage Vesicles: Molecular Architecture, Regulatory Networks, and Their Disruption in Insulin Resistance.International journal of molecular sciences · 2025Review
- A high-content endogenous GLUT4 trafficking assay reveals new aspects of adipocyte biology.Life science alliance · 2023Article
- Knockout of syntaxin-4 in 3T3-L1 adipocytes reveals new insight into GLUT4 trafficking and adiponectin secretion.Journal of cell science · 2022Article
- The Role of Insulin Regulated Aminopeptidase in Endocytic Trafficking and Receptor Signaling in Immune Cells.Frontiers in molecular biosciences · 2020Review
- GLUT4 Storage Vesicles: Specialized Organelles for Regulated Trafficking.The Yale journal of biology and medicine · 2019Review
- Trans-Modulation of the Somatostatin Type 2A Receptor Trafficking by Insulin-Regulated Aminopeptidase Decreases Limbic Seizures.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Coordinated Regulation of Vasopressin Inactivation and Glucose Uptake by Action of TUG Protein in Muscle.The Journal of biological chemistry · 2015Article
- EARP is a multisubunit tethering complex involved in endocytic recycling.Nature cell biology · 2015Article
- Intracellular Transport Routes for MHC I and Their Relevance for Antigen Cross-Presentation.Frontiers in immunology · 2015Review
- Dynamic GLUT4 sorting through a syntaxin-6 compartment in muscle cells is derailed by insulin resistance-causing ceramide.Biology open · 2014Article
- Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45.Molecular biology of the cell · 2013Article
- Regulation of integrin endocytic recycling and chemotactic cell migration by syntaxin 6 and VAMP3 interaction.Journal of cell science · 2012Article
- Regulation of intracellular membrane trafficking and cell dynamics by syntaxin-6.Bioscience reports · 2012Review
- Endoproteolytic cleavage of TUG protein regulates GLUT4 glucose transporter translocation.The Journal of biological chemistry · 2012Article
- The role of insulin-regulated aminopeptidase in MHC class I antigen presentation.Frontiers in immunology · 2012Article
- The role of multifunctional M1 metallopeptidases in cell cycle progression.Annals of botany · 2011Review
- Article
- Biogenesis and regulation of insulin-responsive vesicles containing GLUT4.Current opinion in cell biology · 2010Review
- The catalytic and protein-protein interaction domains are required for APM1 function.Plant physiology · 2010Article
- Syntaxin 6 and CAL mediate the degradation of the cystic fibrosis transmembrane conductance regulator.Molecular biology of the cell · 2010Article
Corrections and comments
- Erratum issuedHou, June C [added]
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Insulin recruits two transmembrane proteins, GLUT4 and IRAP, to the plasma membrane of muscle cells and adipocytes. The subcellular trafficking and localization of GLUT4, and to a lesser extent IRAP, have been intensely studied, yet the molecular mechanisms responsible for their insulin-responsive compartmentalization remain unknown. Herein we have investigated the endocytosis and recycling of IRAP from the cell surface back to the insulin-responsive compartment (IRC). Our results show that a key dileucine motif at position 76,77 (LL76,77), although required for the initial biosynthetic entry of IRAP into the IRC, is dispensable for entry into the IRC via the endosomal system. Indeed, we found that an AA76,77 mutant of IRAP is fully capable of undergoing endocytosis and is correctly routed back to the IRC. To verify that the AA76,77 mutant enters the bona fide IRC, we show that the internalized IRAP-AA76,77 construct is sequestered in an IRC that is insensitive to brefeldin A yet sensitive to a dominant-interfering mutant of AS160 (AS160-4P). In addition, we show that the GGA clathrin adaptors are not required for the re-entry of IRAP from the cell surface back into the IRC, whereas the Q-SNARE syntaxin 6 is required for this process.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.