ArticleStem cells (Dayton, Ohio)2008
Assessment of stromal-derived inducing activity in the generation of dopaminergic neurons from human embryonic stem cells.
Article in Stem cells (Dayton, Ohio), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 87 citations in OpenAlex.
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- Proneurogenic actions of follicle-stimulating hormone on neurospheres derived from ovarian cortical cells in vitro.BMC veterinary research · 2024Article
- Directional induction of neural stem cells, a new therapy for neurodegenerative diseases and ischemic stroke.Cell death discovery · 2023Review
- Development, wiring and function of dopamine neuron subtypes.Nature reviews. Neuroscience · 2023Review
- Article
- The future of stem cell therapies for Parkinson disease.Nature reviews. Neuroscience · 2020Review
- Replacing what's lost: a new era of stem cell therapy for Parkinson's disease.Translational neurodegeneration · 2020Review
- Stem cell colony interspacing effect on differentiation to neural cells.Journal of tissue engineering and regenerative medicine · 2018Article
- Self-regulatory factors of embryonic stem cells in co-culture with stromal cells enhance neural differentiation.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Transplantation in the nonhuman primate MPTP model of Parkinson's disease: update and perspectives.Primate biology · 2017Review
- In Vitro Models for Neurogenesis.Cold Spring Harbor perspectives in biology · 2016Review
- Molecular Analysis of Stromal Cells-Induced Neural Differentiation of Mouse Embryonic Stem Cells.PloS one · 2016Article
- The significance of membrane fluidity of feeder cell-derived substrates for maintenance of iPS cell stemness.Scientific reports · 2015Article
- Review
- Stem cells from human exfoliated deciduous tooth exhibit stromal-derived inducing activity and lead to generation of neural crest cells from human embryonic stem cells.Cell journal · 2015Article
- Functional differentiation of midbrain neurons from human cord blood-derived induced pluripotent stem cells.Stem cell research & therapy · 2014Article
- Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS.Frontiers in cell and developmental biology · 2014Article
- Highly efficient differentiation of neural precursors from human embryonic stem cells and benefits of transplantation after ischemic stroke in mice.Stem cell research & therapy · 2013Article
- Lignin Induces ES Cells to Differentiate into Neuroectodermal Cells through Mediation of the Wnt Signaling Pathway.PloS one · 2013Article
- Immortalization of neuronal progenitors using SV40 large T antigen and differentiation towards dopaminergic neurons.Journal of cellular and molecular medicine · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Producing dopaminergic (DA) neurons is a major goal of human embryonic stem cell (hESC) research. DA neurons can be differentiated from hESC by coculture with the mouse PA6 stromal cell line; this differentiation-inducing effect is termed stromal-derived inducing activity (SDIA). The molecular and biochemical nature of SDIA is, however, unknown. Various studies have suggested that SDIA involves either a fixation-resistant component located on the PA6 cell surface or factors secreted into the medium by PA6 cells. To address this question, hESC were cocultured with PA6 cells for 12 days and then further differentiated with sonic hedgehog homolog, fibroblast growth factor-8, and glial cell line-derived neurotrophic factor. After 18 days, 34% of cells were tyrosine hydroxylase (TH)+. When PA6 cells were fixed or irradiated, the number of TH+ cells was decreased by threefold, whereas mitomycin-c treatment of feeder cells decreased the number of TH+ cells by 32%. The neural-inducing effect of PA6 cells, as monitored by beta-III-tubulin expression, was minimally affected by mitomycin-c treatment or fixation but was decreased 50% by irradiation. Medium conditioned by PA6 cells was ineffective in differentiating TH+ cells when used alone. Conditioned medium combined with heparin and/or fixed PA6 cells produced TH+ cell differentiation, although less effectively than PA6 cell coculture. Thus, PA6 cell surface activity is required for neural differentiation of hESC, but secreted factors are required for the specific DA neuron-inducing effect. Disclosure of potential conflicts of interest is found at the end of this article.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.