ArticleThe Journal of biological chemistry2008
Transforming growth factor beta up-regulates cysteine-rich protein 2 in vascular smooth muscle cells via activating transcription factor 2.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Single-cell spatial transcriptomics unravels the cellular landscape of abdominal aortic aneurysm.JCI insight · 2025Article
- TGF-β signaling promotes desmoid tumor formation via CSRP2 upregulation.Cancer science · 2024Article
- Dual-Specificity Phosphatase 6 Deficiency Attenuates Arterial-Injury-Induced Intimal Hyperplasia in Mice.International journal of molecular sciences · 2023Article
- Cysteine-rich protein 2 deficiency attenuates angiotensin II-induced abdominal aortic aneurysm formation in mice.Journal of biomedical science · 2022Article
- Kidney single-cell transcriptome profile reveals distinct response of proximal tubule cells to SGLT2i and ARB treatment in diabetic mice.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- CSRP2 suppresses colorectal cancer progressionTheranostics · 2020Article
- The TGFβ superfamily in cardiac dysfunction.Acta biochimica et biophysica Sinica · 2018Review
- Inflammation and vascular smooth muscle cell dedifferentiation following carotid artery ligation.Physiological genomics · 2017Article
- Regulatory RNAs controlling vascular (dys)function by affecting TGF-ß family signalling.EXCLI journal · 2015Review
- Smooth muscle phenotype switching in blast traumatic brain injury-induced cerebral vasospasm.Translational stroke research · 2014Review
- Divergent signaling pathways cooperatively regulate TGFβ induction of cysteine-rich protein 2 in vascular smooth muscle cells.Cell communication and signaling : CCS · 2014Article
- Can proteomics yield insight into aging aorta?Proteomics. Clinical applications · 2013Review
- Phosphorylation of ATF2 and interaction with NFY induces c-Jun in the gonadotrope.Molecular and cellular endocrinology · 2013Article
- KLF5 and hhLIM cooperatively promote proliferation of vascular smooth muscle cells.Molecular and cellular biochemistry · 2012Article
- ATF2 - at the crossroad of nuclear and cytosolic functions.Journal of cell science · 2012Article
- Intronic CArG box regulates cysteine-rich protein 2 expression in the adult but not in developing vasculature.Arteriosclerosis, thrombosis, and vascular biology · 2010Article
- Emerging roles of ATF2 and the dynamic AP1 network in cancer.Nature reviews. Cancer · 2010Review
- "FRNKly, smooth muscle, I don't give a CArG!": a novel mechanism for smooth muscle cell differentiation.Arteriosclerosis, thrombosis, and vascular biology · 2008Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
CRP2 (cysteine-rich protein) is a vascular smooth muscle cell (VSMC)-expressed LIM-only protein. CRP2 associates with the actin cytoskeleton and interacts with transcription factors in the nucleus to mediate smooth muscle cell gene expression. Using Csrp2 (gene symbol of the mouse CRP2 gene)-deficient mice, we previously demonstrated that an absence of CRP2 enhances VSMC migration and increases neointima formation following arterial injury. Despite its importance in vascular injury, the molecular mechanisms controlling CRP2 expression in VSMC are largely unknown. Transforming growth factor beta (TGFbeta), a key factor present in the vessel wall in the early phases of arterial response to injury, plays an important role in modulating lesion formation. Because both CRP2 and TGFbeta are mediators of VSMC responses, we examined the possibility that TGFbeta might regulate CRP2 expression. TGFbeta significantly induced CRP2 mRNA and protein expression in VSMCs. Promoter analysis identified a conserved cAMP-responsive element (CRE)-like site (TAACGTCA) in the Csrp2 promoter that was critical for basal promoter activity and response to TGFbeta. Gel mobility shift assays revealed that mainly ATF2 bound to this CRE-like element, and mutation of the CRE sequences abolished binding. TGFbeta enhanced the activation of ATF2, leading to increased phospho-ATF2 levels within the DNA-protein complexes. Furthermore, ATF2-transactivated Csrp2 promoter activity and TGFbeta enhanced this activation. In addition, a phosphorylation-negative ATF2 mutant construct decreased basal and TGFbeta-mediated Csrp2 promoter activity. Our results show for the first time in VSMC that TGFbeta activates ATF2 phosphorylation and Csrp2 gene expression via a CRE promoter element.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.