ArticleJournal of structural biology2008
Quantitative 3-D imaging of eukaryotic cells using soft X-ray tomography.
Article in Journal of structural biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 158 citations in OpenAlex.
- Laboratory-based cellular-level correlative visible-light and X-ray microscopy for 3D evaluation of mouse kidney biopsy.Scientific reports · 2026Article
- Cryo-CLXEM introduces cryo-SXT to bridge the resolution gap in cryo-CLEM.Communications biology · 2025Article
- Automated segmentation of soft X-ray tomography: Native cellular structure with submicron resolution at high-throughput for whole-cell quantitative imaging in yeast.Molecular biology of the cell · 2025Article
- Observation of biological and emulsion samples by newly developed three-dimensional impedance scanning electron microscopy.Computational and structural biotechnology journal · 2024Article
- 3D surface reconstruction of cellular cryo-soft X-ray microscopy tomograms using semisupervised deep learning.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Vast heterogeneity in cytoplasmic diffusion rates revealed by nanorheology and Doppelgänger simulations.Biophysical journal · 2023Article
- An intensity-based post-processing tool for 3D instance segmentation of organelles in soft X-ray tomograms.PloS one · 2022Article
- Synchrotron multimodal imaging in a whole cell reveals lipid droplet core organization.Journal of synchrotron radiation · 2020Article
- The cellular landscape by cryo soft X-ray tomography.Biophysical reviews · 2019Review
- Imaging cell morphology and physiology using X-rays.Biochemical Society transactions · 2019Review
- Article
- Lensless Tomographic Imaging of Near Surface Structures of Frozen Hydrated Malaria-Infected Human Erythrocytes by Coherent X-Ray Diffraction Microscopy.Scientific reports · 2017Article
- Visualizing red blood cell sickling and the effects of inhibition of sphingosine kinase 1 using soft X-ray tomography.Journal of cell science · 2016Article
- Imaging and characterizing cells using tomography.Archives of biochemistry and biophysics · 2015Review
- Three-dimensional imaging of human stem cells using soft X-ray tomography.Journal of the Royal Society, Interface · 2015Article
- Review of methods to probe single cell metabolism and bioenergetics.Metabolic engineering · 2015Review
- Further closing the resolution gap: integrating cryo-soft x-ray and light microscopies.Biophysical journal · 2014Article
- Imaging endosomes and autophagosomes in whole mammalian cells using correlative cryo-fluorescence and cryo-soft X-ray microscopy (cryo-CLXM).Ultramicroscopy · 2014Article
- Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Imaging has long been one of the principal techniques used in biological and biomedical research. Indeed, the field of cell biology grew out of the first electron microscopy images of organelles in a cell. Since this landmark event, much work has been carried out to image and classify the organelles in eukaryotic cells using electron microscopy. Fluorescently labeled organelles can now be tracked in live cells, and recently, powerful light microscope techniques have pushed the limit of optical resolution to image single molecules. In this paper, we describe the use of soft X-ray tomography, a new tool for quantitative imaging of organelle structure and distribution in whole, fully hydrated eukaryotic Schizosaccharomyces pombe cells. In addition to imaging intact cells, soft X-ray tomography has the advantage of not requiring the use of any staining or fixation protocols--cells are simply transferred from their growth environment to a sample holder and immediately cryofixed. In this way the cells can be imaged in a near native state. Soft X-ray tomography is also capable of imaging relatively large numbers of cells in a short period of time, and is therefore a technique that has the potential to produce information on organelle morphology from statistically significant numbers of cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.