ArticleAnalytical chemistry2008
PCR-free quantitative detection of genetically modified organism from raw materials. An electrochemiluminescence-based bio bar code method.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- Optical Image Sensors for Smart Analytical Chemiluminescence Biosensors.Bioengineering (Basel, Switzerland) · 2024Review
- Detection ofRSC advances · 2018Article
- A high-sensitivity optical device for the early monitoring of plant pathogen attack via the in vivo detection of ROS bursts.Frontiers in plant science · 2015Article
- Synthesis, labeling and bioanalytical applications of a tris(2,2'-bipyridyl)ruthenium(II)-based electrochemiluminescence probe.Nature protocols · 2014Article
- Ligand synthesis and passivation for silver and large gold nanoparticles for single-particle-based sensing and spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Label-free electrochemical detection of the specific oligonucleotide sequence of dengue virus type 1 on pencil graphite electrodes.Sensors (Basel, Switzerland) · 2011Article
- Study on a luminol-based electrochemiluminescent sensor for label-free DNA sensing.Sensors (Basel, Switzerland) · 2010Article
- PCR-free detection of genetically modified organisms using magnetic capture technology and fluorescence cross-correlation spectroscopy.PloS one · 2009Article
- Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction.BMC genomics · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
A bio bar code assay based on oligonucleotide-modified gold nanoparticles (Au-NPs) provides a PCR-free method for quantitative detection of nucleic acid targets. However, the current bio bar code assay requires lengthy experimental procedures including the preparation and release of bar code DNA probes from the target-nanoparticle complex and immobilization and hybridization of the probes for quantification. Herein, we report a novel PCR-free electrochemiluminescence (ECL)-based bio bar code assay for the quantitative detection of genetically modified organism (GMO) from raw materials. It consists of tris-(2,2'-bipyridyl) ruthenium (TBR)-labeled bar code DNA, nucleic acid hybridization using Au-NPs and biotin-labeled probes, and selective capture of the hybridization complex by streptavidin-coated paramagnetic beads. The detection of target DNA is realized by direct measurement of ECL emission of TBR. It can quantitatively detect target nucleic acids with high speed and sensitivity. This method can be used to quantitatively detect GMO fragments from real GMO products.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.