ArticleJournal of clinical biochemistry and nutrition2008
Saposin B is a human coenzyme q10-binding/transfer protein.
Article in Journal of clinical biochemistry and nutrition, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Blood-Brain Barrier and Neuronal Model Systems for Studying CoQAntioxidants (Basel, Switzerland) · 2025Review
- Coenzyme Q10 and the Blood-Brain Barrier: An Overview.Journal of clinical medicine · 2025Review
- Understanding coenzyme Q.Physiological reviews · 2024Review
- Resveratrol is converted to the ring portion of coenzyme Q10 and raises intracellular coenzyme Q10 levels in HepG2 cell.Journal of clinical biochemistry and nutrition · 2024Article
- Prosaposin is a novel coenzyme Q10-binding protein.Journal of clinical biochemistry and nutrition · 2024Article
- New Insights on the Uptake and Trafficking of Coenzyme Q.Antioxidants (Basel, Switzerland) · 2023Review
- Riboflavin compounds show NAD(P)H dependent quinone oxidoreductase-like quinone reducing activity.Journal of clinical biochemistry and nutrition · 2023Article
- Coenzyme Q10 Metabolism: A Review of Unresolved Issues.International journal of molecular sciences · 2023Review
- Cellular level of coenzyme Q increases with neuronal differentiation, playing an important role in neural elongations.Journal of clinical biochemistry and nutrition · 2022Article
- Reduced prosaposin levels in HepG2 cells with long-term coenzyme Q10 deficiency.Journal of clinical biochemistry and nutrition · 2022Article
- Production of Coenzyme QWorld journal of microbiology & biotechnology · 2022Review
- Coenzyme Q10 levels increase with embryonic development in medaka.Journal of clinical biochemistry and nutrition · 2022Article
- Coenzyme Q10, Ageing and the Nervous System: An Overview.Antioxidants (Basel, Switzerland) · 2021Review
- Opisthorchis felineus genes differentially expressed under praziquantel shed light on the nature of tegument disruption and indicate the adaptive role of cGMP-dependent protein kinase.Parasitology research · 2020Article
- Bioavailability of Coenzyme QAntioxidants (Basel, Switzerland) · 2020Review
- Mitochondrial adaptation in human mesenchymal stem cells following ionizing radiation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Increased oxidative stress and renal injury in patients with sepsis.Journal of clinical biochemistry and nutrition · 2018Article
- Increased oxidative stress and coenzyme Q10 deficiency in centenarians.Journal of clinical biochemistry and nutrition · 2018Article
- Idebenone and coenzyme QDisease models & mechanisms · 2018Article
- Exploring the Multiligand Binding Specificity of Saposin B Reveals Two Binding Sites.ACS omega · 2017Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coenzyme Q10 (CoQ10) is essential for ATP production in the mitochondria, and is an important antioxidant in every biomembrane and lipoprotein. Due to its hydrophobicity, a binding and transfer protein for CoQ10 is plausible, but none have yet been isolated and characterized. Here we purified a CoQ10-binding protein from human urine and identified it to be saposin B, a housekeeping protein necessary for sphingolipid hydrolysis in lysosomes. We confirmed that cellular saposin B binds CoQ10 in human sperm and the hepatoma cell line HepG2 by using saposin B monoclonal antibody. The molar ratios of CoQ10 to saposin B were estimated to be 0.22 in urine, 0.003 in HepG2, and 0.12 in sperm. We then confirmed that aqueous saposin B extracts CoQ10 from hexane to form a saposin B-CoQ10 complex. Lipid binding affinity to saposin B decreased in the following order: CoQ10>CoQ9>CoQ7>>alpha-tocopherol>>cholesterol (no binding). The CoQ10-binding affinity to saposin B increased with pH, with maximal binding seen at pH 7.4. On the other hand, the CoQ10-donating activity of the saposin B-CoQ10 complex to erythrocyte ghost membranes increased with decreasing pH. These results suggest that saposin B binds and transports CoQ10 in human cells.
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