ArticleBiological procedures online2008
Optimized two-dimensional thin layer chromatography to monitor the intracellular concentration of acetyl phosphate and other small phosphorylated molecules.
Article in Biological procedures online, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The trial behind it
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Who cites it
15 citing papers in PubMed.
- Oxidative stress-induced CDO1 glutathionylation regulates cysteine metabolism and sustains redox homeostasis under ionizing radiation.Redox biology · 2025Article
- CDO1 phosphorylation is required for IL-6-induced tumor cell proliferation through governing cysteine availability.Cell communication and signaling : CCS · 2025Article
- Acetylation of NarL K188 and K192 is involved in regulating Escherichia coli anaerobic nitrate respiration.Applied microbiology and biotechnology · 2022Article
- Post-translational Protein Acetylation: An Elegant Mechanism for Bacteria to Dynamically Regulate Metabolic Functions.Frontiers in microbiology · 2019Review
- Metabolic intermediate acetyl phosphate modulates bacterial virulence via acetylation.Emerging microbes & infections · 2019Article
- Reversible lysine acetylation is involved in DNA replication initiation by regulating activities of initiator DnaA in Escherichia coli.Scientific reports · 2016Article
- In vitro evidence that RNA Polymerase acetylation and acetyl phosphate-dependent CpxR phosphorylation affect cpxP transcription regulation.FEMS microbiology letters · 2016Article
- The Borrelia burgdorferi RelA/SpoT Homolog and Stringent Response Regulate Survival in the Tick Vector and Global Gene Expression during Starvation.PLoS pathogens · 2015Article
- Genetics and Physiology of Acetate Metabolism by the Pta-Ack Pathway of Streptococcus mutans.Applied and environmental microbiology · 2015Article
- Structural, kinetic and proteomic characterization of acetyl phosphate-dependent bacterial protein acetylation.PloS one · 2014Article
- Inhibition of acetyl phosphate-dependent transcription by an acetylatable lysine on RNA polymerase.The Journal of biological chemistry · 2012Article
- Evaluation of protocols used in 2-d electrophoresis for proteome analysis of young rice caryopsis.Genomics, proteomics & bioinformatics · 2011Article
- Physiologically relevant small phosphodonors link metabolism to signal transduction.Current opinion in microbiology · 2010Review
- E Unibus Plurum: genomic analysis of an experimentally evolved polymorphism in Escherichia coli.PLoS genetics · 2009Article
- Signal integration by the two-component signal transduction response regulator CpxR.Journal of bacteriology · 2008Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Acetyl phosphate (acetyl-P) serves critical roles in coenzyme A recycling and ATP synthesis. It is the intermediate of the Pta-AckA pathway that inter-converts acetyl-coenzyme A and acetate. Acetyl-P also can act as a global signal by donating its phosphoryl group to specific two-component response regulators. This ability derives from its capacity to store energy in the form of a high-energy phosphate bond. This bond, while critical to its function, also destabilizes acetyl-P in cell extracts. This lability has greatly complicated biochemical analysis, leading in part to widely varying acetyl-P measurements. We therefore developed an optimized protocol based on two-dimensional thin layer chromatography that includes metabolic labeling under aerated conditions and careful examination of the integrity of acetyl-P within extracts. This protocol results in greatly improved reproducibility, and thus permits precise measurements of the intracellular concentration of acetyl-P, as well as that of other small phosphorylated molecules.
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Registered trials
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