ArticleMolecular biology of the cell2008
A supraphysiological nuclear export signal is required for parvovirus nuclear export.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Nuclear entry and egress of parvoviruses.Molecular microbiology · 2022Review
- G2/M checkpoint regulation and apoptosis facilitate the nuclear egress of parvoviral capsids.Frontiers in cell and developmental biology · 2022Article
- Human Adenovirus Type 5 Infection Leads to Nuclear Envelope Destabilization and Membrane Permeability Independently of Adenovirus Death Protein.International journal of molecular sciences · 2021Article
- Concepts to Reveal Parvovirus-Nucleus Interactions.Viruses · 2021Review
- Cancer Therapy with Nanoparticle-Medicated Intracellular Expression of Peptide CRM1-Inhibitor.International journal of nanomedicine · 2021Article
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- Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals.eLife · 2019Article
- Correlation of CRM1-NES affinity with nuclear export activity.Molecular biology of the cell · 2018Article
- Intracellular Localization ofViruses · 2018Article
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- Protoparvovirus Knocking at the Nuclear Door.Viruses · 2017Review
- Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export.Journal of virology · 2017Article
- Late Maturation Steps Preceding Selective Nuclear Export and Egress of Progeny Parvovirus.Journal of virology · 2016Article
- Reorganization of Nuclear Pore Complexes and the Lamina in Late-Stage Parvovirus Infection.Journal of virology · 2015Article
- Oncolytic parvoviruses: from basic virology to clinical applications.Virology journal · 2015Review
- Complementation for an essential ancillary non-structural protein function across parvovirus genera.Virology · 2014Article
- Chikungunya virus capsid protein contains nuclear import and export signals.Virology journal · 2013Article
- An in-frame deletion in the NS protein-coding sequence of parvovirus H-1PV efficiently stimulates export and infectivity of progeny virions.Journal of virology · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRM1 exports proteins that carry a short leucine-rich peptide signal, the nuclear export signal (NES), from the nucleus. Regular NESs must have low affinity for CRM1 to function optimally. We previously generated artificial NESs with higher affinities for CRM1, termed supraphysiological NESs. Here we identify a supraphysiological NES in an endogenous protein, the NS2 protein of parvovirus Minute Virus of Mice (MVM). NS2 interacts with CRM1 without the requirement of RanGTP, whereas addition of RanGTP renders the complex highly stable. Mutation of a single hydrophobic residue that inactivates regular NESs lowers the affinity of the NS2 NES for CRM1 from supraphysiological to regular. Mutant MVM harboring this regular NES is compromised in viral nuclear export and productivity. In virus-infected mouse fibroblasts we observe colocalization of NS2, CRM1 and mature virions, which is dependent on the supraphysiological NS2 NES. We conclude that supraphysiological NESs exist in nature and that the supraphysiological NS2 NES has a critical role in active nuclear export of mature MVM particles before cell lysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.