ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2008
Presynaptic calcium channel localization and calcium-dependent synaptic vesicle exocytosis regulated by the Fuseless protein.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- PTPN11/Corkscrew Activates Local Presynaptic Mapk Signaling to Regulate Synapsin, Synaptic Vesicle Pools, and Neurotransmission Strength, with a Dual Requirement in Neurons and Glia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Monomeric α-synuclein activates the plasma membrane calcium pump.The EMBO journal · 2023Article
- FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation.Journal of visualized experiments : JoVE · 2018Article
- From damage response to action potentials: early evolution of neural and contractile modules in stem eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Dynamic regulation of mRNA decay during neural development.Neural development · 2015Article
- Molecular underpinnings of synaptic vesicle pool heterogeneity.Traffic (Copenhagen, Denmark) · 2015Review
- Two protein N-acetylgalactosaminyl transferases regulate synaptic plasticity by activity-dependent regulation of integrin signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- N-glycosylation requirements in neuromuscular synaptogenesis.Development (Cambridge, England) · 2013Article
- The role of Drosophila cytidine monophosphate-sialic acid synthetase in the nervous system.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- The cell polarity scaffold Lethal Giant Larvae regulates synapse morphology and function.Journal of cell science · 2013Article
- Glycosylated synaptomatrix regulation of trans-synaptic signaling.Developmental neurobiology · 2012Review
- Extracellular matrix and its receptors in Drosophila neural development.Developmental neurobiology · 2011Review
- The Cdc42-selective GAP rich regulates postsynaptic development and retrograde BMP transsynaptic signaling.The Journal of cell biology · 2010Article
- The nonsense-mediated decay pathway maintains synapse architecture and synaptic vesicle cycle efficacy.Journal of cell science · 2010Article
- Sialyltransferase regulates nervous system function in Drosophila.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Loose coupling between calcium channels and sites of exocytosis in chromaffin cells.The Journal of physiology · 2009Article
- Presynaptic CaV2 calcium channel traffic requires CALF-1 and the alpha(2)delta subunit UNC-36.Nature neuroscience · 2009Article
- Maturation of active zone assembly by Drosophila Bruchpilot.The Journal of cell biology · 2009Article
- Metabotropic glutamate receptor-mediated use-dependent down-regulation of synaptic excitability involves the fragile X mental retardation protein.Journal of neurophysiology · 2009Article
- Cav2-type calcium channels encoded by cac regulate AP-independent neurotransmitter release at cholinergic synapses in adult Drosophila brain.Journal of neurophysiology · 2009Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
A systematic forward genetic Drosophila screen for electroretinogram mutants lacking synaptic transients identified the fuseless (fusl) gene, which encodes a predicted eight-pass transmembrane protein in the presynaptic membrane. Null fusl mutants display >75% reduction in evoked synaptic transmission but, conversely, an approximately threefold increase in the frequency and amplitude of spontaneous synaptic vesicle fusion events. These neurotransmission defects are rescued by a wild-type fusl transgene targeted only to the presynaptic cell, demonstrating a strictly presynaptic requirement for Fusl function. Defects in FM dye turnover at the synapse show a severely impaired exo-endo synaptic vesicle cycling pool. Consistently, ultrastructural analyses reveal accumulated vesicles arrested in clustered and docked pools at presynaptic active zones. In the absence of Fusl, calcium-dependent neurotransmitter release is dramatically compromised and there is little enhancement of synaptic efficacy with elevated external Ca(2+) concentrations. These defects are causally linked with severe loss of the Cacophony voltage-gated Ca(2+) channels, which fail to localize normally at presynaptic active zone domains in the absence of Fusl. These data indicate that Fusl regulates assembly of the presynaptic active zone Ca(2+) channel domains required for efficient coupling of the Ca(2+) influx and synaptic vesicle exocytosis during neurotransmission.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.