ArticleThe Journal of biological chemistry2008
Dual specificity phosphatases 18 and 21 target to opposing sides of the mitochondrial inner membrane.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
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- Dusp26 phosphatase regulates mitochondrial respiration and oxidative stress and protects neuronal cell death.Cellular and molecular life sciences : CMLS · 2022Article
- Tissue microRNA expression profiling in hepatic and pulmonary metastatic melanoma.Melanoma research · 2020Article
- Mitochondrial Enzymes of the Urea Cycle Cluster at the Inner Mitochondrial Membrane.Frontiers in physiology · 2020Article
- Ptc7p Dephosphorylates Select Mitochondrial Proteins to Enhance Metabolic Function.Cell reports · 2017Article
- MS1 Peptide Ion Intensity Chromatograms in MS2 (SWATH) Data Independent Acquisitions. Improving Post Acquisition Analysis of Proteomic Experiments.Molecular & cellular proteomics : MCP · 2015Article
- Hallmarks of a new era in mitochondrial biochemistry.Genes & development · 2013Review
- Mitochondrial phosphorylation in apoptosis: flipping the death switch.Antioxidants & redox signaling · 2013Review
- Cardiac mitochondrial matrix and respiratory complex protein phosphorylation.American journal of physiology. Heart and circulatory physiology · 2012Review
- Mitochondrial phosphatase PTPMT1 is essential for cardiolipin biosynthesis.Cell metabolism · 2011Article
- High throughput microplate respiratory measurements using minimal quantities of isolated mitochondria.PloS one · 2011Article
- Glutathione peroxidase 4 differentially regulates the release of apoptogenic proteins from mitochondria.Free radical biology & medicine · 2009Article
- Monitoring phosphorylation of the pyruvate dehydrogenase complex.Analytical biochemistry · 2009Article
- Redox signaling and protein phosphorylation in mitochondria: progress and prospects.Journal of bioenergetics and biomembranes · 2009Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Although large-scale approaches have identified numerous mitochondrial phosphoproteins, little is known about the mitochondrial kinases and phosphatases that regulate these phosphoproteins. Here, we identify two members of the atypical dual specificity phosphatases (DSP), DSP18 and DSP21, that are localized in mitochondria. Although DSP18 is widely expressed in several mammalian tissues, DSP21 is selectively expressed in the testes. We demonstrate that DSP18 and DSP21 are targeted to mitochondria by cryptic internal localization signals. Subfractionation of mitochondria demonstrated that DSP18 is located in the intermembrane space as a peripheral membrane protein of the inner membrane. In contrast, subfractionation of rat testis mitochondria revealed DSP21 is localized to the matrix as a peripheral membrane protein of the inner membrane. Moreover, we demonstrate that a previously reported substrate for DSP18, the stress-activated protein kinase, does not localize to mitochondria in several different tissues, making it an unlikely substrate for DSP18. Finally, we show that induction of apoptosis by treatment with staurosporine causes translocation of DSP18 from the intermembrane space into the cytosol similar to other apoptogenic factors such as cytochrome c. This work rigorously demonstrates the unique location of two highly similar DSPs on opposing sides of the mitochondrial inner membrane.
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Registered trials
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