ArticleHuman reproduction (Oxford, England)2008
Restricted expression of the human DAZ protein in premeiotic germ cells.
Article in Human reproduction (Oxford, England), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Y-chromosome genes associated with sertoli cell-only syndrome identified by array comparative genome hybridization.Biomedical journal · 2023Article
- BOULE, a Deleted in Azoospermia Homolog, Is Recruited to Stress Granules in the Mouse Male Germ Cells.PloS one · 2016Article
- Article
- Mouse sperm acquire a new structure on the apical hook during epididymal maturation.Asian journal of andrology · 2013Article
- The roles of the DAZ family in spermatogenesis: More than just translation?Spermatogenesis · 2011Article
- Azoospermia factor and male infertility.Reproductive medicine and biology · 2010Review
- Increased apoptosis of germ cells in patients with AZFc deletions.Journal of assisted reproduction and genetics · 2010Article
- Genetic dissection of the AZF regions of the human Y chromosome: thriller or filler for male (in)fertility?Journal of biomedicine & biotechnology · 2010Review
- Gene expression profiles of mouse spermatogenesis during recovery from irradiation.Reproductive biology and endocrinology : RB&E · 2009Article
- Boule is present in fish and bisexually expressed in adult and embryonic germ cells of medaka.PloS one · 2009Article
- The AZFc region of the Y chromosome: at the crossroads between genetic diversity and male infertility.Human reproduction updateReview
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundThe role of the Y chromosome-encoded Deleted in Azoospermia (DAZ) gene family in spermatogenesis remains unclear. The ability of men without the DAZ gene to produce sperm, as well as the lack of selective pressure on DAZ exon sequences during evolution, casts doubts on its functional significance. Most men have four DAZ genes encoding protein isoforms that differ significantly in size. However, published western blots showed only a single "DAZ" band, raising the possibility that not all four DAZ genes are expressed.
methodsRT-PCR, western blotting and immunostaining were used to study the expression of the four DAZ genes and the autosomal DAZL gene in human testes and in tissue culture cells.
resultsRNA transcripts of all four DAZ genes were found in the testis, but at much lower levels than that of the DAZL transcripts. Expression in cultured somatic cells showed that DAZ transcripts encoding multiple DAZ repeats were translated inefficiently. No DAZ proteins could be unambiguously identified on western blots when the testicular samples from three patients without the DAZ genes were used as negative controls. Nonetheless, low levels of DAZ were detected in the cytoplasm of spermatogonia by immunostaining.
conclusionsThe expression of DAZ proteins in adult human testes is restricted to the spermatogonia and suggests a premeiotic role.
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